TCDD-inducible poly(ADP-ribose) polymerase:: A novel response to 2,3,7,8-tetrachlorodibenzo-p-dioxin
TCDD-inducible poly(ADP-ribose) polymerase:: A novel response to 2,3,7,8-tetrachlorodibenzo-p-dioxin
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DOI：
10.1006/bbrc.2001.5987
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发表时间：
2001-11-30
影响因子：
3.1
通讯作者：
Dong, LQ
中科院分区：
文献类型：
--
作者：
Ma, Q;Baldwin, KT;Dong, LQ
2，3，7，8-四氯二苯并-对-二恶英（TCDD）通过调节基因表达在哺乳动物中引起多效性效应。在这里，我们分析了TCDD诱导的mRNA表达的mRNA差异显示和报告的一种新的TCDD诱导的聚（ADP-核糖）聚合酶（TiPARP）的克隆。TiPARP cDNA含有657个氨基酸残基的开放阅读框;羧基半共享与PARP的催化结构域的序列相似性，PARP是催化蛋白质的聚ADP-核糖基化的酶家族。通过体外转录/翻译的cDNA表达揭示了类似于75 kDa的蛋白质。表达的TiPARP对组蛋白表现出PARP活性。TiPARP与在长时程增强（一种记忆形成过程）期间诱导的RM 1和在浸润进行性肿瘤的T细胞中诱导的TIL高度同源。TiPARP mRNA在广泛的小鼠组织中表达。总之，这些数据表明TiPARP是TCDD的一种新靶点，其可能通过聚ADP-核糖基化调节蛋白质功能而促进对TCDD的多种应答。(C)2001年，爱思唯尔科学。
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes pleotropic effects in mammalian species through modulating gene expression. Here we analyzed TCDD-induced mRNA expression by using mRNA differential display and report the cloning of a novel TCDD-inducible poly(ADP-ribose) polymerase (TiPARP). TiPARP cDNA contains an open reading frame of 657 amino acid residues; the carboxyl half shares sequence similarity to the catalytic domain of PARP, a family of enzymes that catalyze poly ADP-ribosylation of proteins. Expression of the cDNA by in vitro transcription/translation reveals a protein of similar to 75 kDa. The expressed TiPARP exhibits PARP activity toward histone. TiPARP is highly homologous to RM1 which is induced during long-term potentiation, a memory formation process, and to TIL which is induced in T cells infiltrating progressing tumors. TiPARP mRNA is expressed in a broad range of mouse tissues. Together, these data demonstrate that TiPARP is a novel target of TCDD that may contribute to multiple responses to TCDD by modulating protein function through Poly ADP-ribosylation. (C) 2001 Elsevier Science.