Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars
Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars
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DOI：
10.1534/genetics.105.044586
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发表时间：
2006-02-01
期刊：
影响因子：
3.3
通讯作者：
Sorrells, ME
中科院分区：
文献类型：
--
作者：
Breseghello, F;Sorrells, ME
关联作图是一种基于连锁不平衡（LD）检测基因效应的方法，它补充了QTL分析在分子植物育种工具开发中的作用。以95个软质冬小麦品种为研究对象，进行了关联捕捉分析。群体结构估计的基础上，36个非连锁的简单重复序列（SSR）标记。LD的程度估计染色体2D和5A的一部分，相对于未连锁标记之间观察到的LD。2D染色体上的LD为1 cM，而在5A的着丝粒区域，LD延伸至5 cM。以2D、5A和5B染色体上的62个SSR标记为随机因子，以待测标记为固定因子，采用混合效应模型分析了SSR标记与籽粒形态和碾磨品质的关联性。排列用于调整染色体内多重检验的显著性阈值。与先前的QTL分析一致，在测试的三条染色体上检测到籽粒大小的显著标记，并鉴定了潜在的用于选择的等位基因。我们的研究结果表明，关联捕捉可以补充和增强以前的QTL信息的标记辅助选择。
Association mapping is a method for detection of gene effects based on linkage disequilibrium (LD) that complements QTL analysis in the development of tools for molecular plant breeding. In this study, association snapping was performed on a selected sample of 95 cultivars of soft winter wheat. Population structure was estimated on the basis of 36 unlinked simple-sequence repeat (SSR) markers. The extent of LD was estimated on chromosomes 2D and part of 5A, relative to the LD observed among unlinked markers. Consistent LD on chromosome 2D was 1 cM, whereas in the centromeric region of 5A, LD extended for similar to 5 cM. Association of 62 SSR loci on chromosomes 2D, 5A, and 5B with kernel morphology and milling quality was analyzed through a mixed-effects model, where subpopulation was considered as a random factor and the marker tested was considered as a fixed factor. Permutations were used to adjust the threshold of significance for multiple testing within chromosomes. In agreement with previous QTL analysis, significant markers for kernel size were detected on the three chromosomes tested, and alleles potentially useful for selection were identified. Our results demonstrated that association snapping could complement and enhance previous QTL information for marker-assisted selection.