SEQUENTIAL INCREASES IN PROLIFERATING CELL NUCLEAR ANTIGEN EXPRESSION IN HEAD AND NECK TUMORIGENESIS - A POTENTIAL BIOMARKER
SEQUENTIAL INCREASES IN PROLIFERATING CELL NUCLEAR ANTIGEN EXPRESSION IN HEAD AND NECK TUMORIGENESIS - A POTENTIAL BIOMARKER
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DOI：
10.1093/jnci/85.12.971
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发表时间：
1993-06-16
期刊：
影响因子：
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通讯作者：
HITTELMAN, WN
中科院分区：
文献类型：
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作者：
SHIN, DM;VORAVUD, N;HITTELMAN, WN
背景资料：增殖细胞核抗原（PCNA）是一种36 kd的核蛋白，其表达与DNA合成和细胞增殖有关。头颈部鳞状细胞癌的发生是一个多步骤的过程，增殖失调是这一过程的一个潜在标志。目的：分析了鳞状细胞癌组织样本中含有癌前病变（增生和/或异型增生）和相邻的正常上皮细胞中的PCNA失调，以更好地了解头颈部肿瘤发展过程中的增殖变化。方法：用抗PCNA单克隆抗体对福尔马林固定、石蜡包埋切片进行免疫组化分析。分析了33例头颈部鳞状细胞癌及其邻近癌前病变的PCNA表达，并与从未接触过一手烟草烟雾的对照组进行了比较。通过半定量评分（0-3级）在三个上皮层（基底层、副基底层和浅表层）中评估PCNA表达。标签行业 并计算PCNA表达的加权平均指数。结果如下：邻近肿瘤的正常上皮细胞比对照组有更多的增殖活性：PCNA表达的加权平均指数在基底层高4倍，在副基底层高6倍。PCNA表达随癌旁正常上皮向增生的进展而增加（P
Background: Proliferating cell nuclear antigen (PCNA) is a 36-kd nuclear protein whose expression is associated with DNA synthesis and cell proliferation. Tumorigenesis in head and neck squamous cell carcinoma is proposed to be a multistep process; dysregulation of proliferation is a potential marker of this process. Purpose: PCNA dysregulation was analyzed in squamous cell carcinoma tissue samples containing premalignant lesions (hyperplasia and/or dysplasia) and in adjacent normal epithelium to better understand proliferative changes during head and neck tumor development. Methods: Immunohistochemical analysis was performed on formalin-fixed, paraffin-embedded sections by using a monoclonal anti-PCNA antibody. PCNA expression was analyzed in 33 head and neck squamous cell carcinomas and in their adjacent premalignant lesions from different sites and compared with that in the control samples, which had never been exposed to first-hand tobacco smoke. PCNA expression was assessed by semiquantitative scoring (scale 0-3) in three epithelial layers (basal, parabasal, and superficial). The labeling ind ex and the weighted mean index of PCNA expression were calculated. Results: Normal epithelium adjacent to the tumor had much more proliferative activity than the controls: The weighted mean index of PCNA expression was fourfold higher in the basal layer and sixfold higher in the parabasal layer. PCNA expression increased as tissues progressed from adjacent normal epithelium to hyperplasia (P