Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078:: Evolutionary relationships to phages from Corynebacterineae
Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078:: Evolutionary relationships to phages from Corynebacterineae
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DOI：
10.1016/j.virol.2008.05.027
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发表时间：
2008-09-01
期刊：
影响因子：
3.7
通讯作者：
Hu, Hui-Yu
中科院分区：
文献类型：
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作者：
Chen, Chang-Lin;Pan, Tzu-Ying;Hu, Hui-Yu
P1201是谷氨酸棒杆菌NCHU 87078的裂解性棒状杆菌。其基因组由70，579个碱基对的线性双链DNA分子组成，具有3 '突出的粘性末端，通常是核苷酸。我们已经确定了69个假定的开放阅读框架，包括三个明显的基因（胸苷酸合成酶，终止酶，和RNR α亚基基因），被内含肽中断。这些内含肽的蛋白质剪接活动在大肠杆菌中被证明。通过SDS-PAGE分离了主要衣壳蛋白和主要尾部蛋白，并通过LC-MS-MS和N-末端序列分析鉴定了三种结构蛋白。生物信息学分析表明，只有约8.7%的推定基因产物与来自黄色短杆菌的裂解性棒状体BFK 20具有实质性的蛋白质序列相似性，这是迄今为止唯一的基因组已测序的棒状体，揭示了P1201基因组不同于BFK 20。P1201的嵌合样基因组表明P1201、戈登氏菌噬菌体GTE 5、分枝杆菌噬菌体和棒杆菌属的几个区域之间广泛的水平基因转移。基因组(C)2008年爱思唯尔公司All rights reserved.
P1201 is a lytic corynephage of Corynebacterium glutamicum NCHU 87078. Its genome consists of a linear double-stranded DNA molecule of 70,579 base pairs, with 3'-protruding cohesive ends often nucleotides. We have identified 69 Putative open reading frames, including three apparent genes (thymidylate synthase, terminase, and RNR alpha subunit genes) that are interrupted by an intein. Protein-splicing activities of these inteins were demonstrated in Escherichia coli. Three structural proteins including major capsid and major tail proteins were separated by SDS-PAGE and identified by both LC-MS-MS and N-terminal sequence analyses. Bioinformatics analysis indicated that only about 8.7% of its putative gene products shared Substantial protein sequence similarity with the lytic corynephage BFK20 from Brevibacterium flavum, the only corynephage whose genome had been sequenced to date, revealing that the P1201 genome is distinct from BFK20. The mosaic-like genome of P1201 indicates extensive horizontal gene transfer among P1201, Gordonia terrae phage GTE5, mycobacteriophages, and several regions of Corynebacterium spp. genomes. (C) 2008 Elsevier Inc. All rights reserved.