Transcriptome profiling of the Plutella xylostella (Lepidoptera: Plutellidae) ovary reveals genes involved in oogenesis
Transcriptome profiling of the Plutella xylostella (Lepidoptera: Plutellidae) ovary reveals genes involved in oogenesis
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小菜蛾（鳞翅目：小菜蛾科）卵巢的转录组分析揭示了参与卵子发生的基因
DOI：
10.1016/j.gene.2017.09.020
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发表时间：
2017-12-30
期刊：
影响因子：
3.5
通讯作者：
You, Min-Sheng
中科院分区：
文献类型：
--
作者：
Peng, Lu;Wang, Lei;You, Min-Sheng
研究背景：昆虫卵巢作为一种特化器官，在维持生殖力和种群生存方面发挥着重要作用。卵子发生是导致成熟卵产生的复杂生理过程，涉及表观遗传编程、生殖细胞行为、细胞周期调控等多个方面。鉴定与卵巢发育和卵子发生相关的基因，对于更好地了解小菜蛾的生殖生物学和筛选潜在的分子靶标具有重要意义。基于转录组测序，总共获得了7.88 Gb的干净核苷酸，鉴定了19，934个基因和1861个新转录本。基因表达谱分析表明，61.7%的基因（FPKM 1）在小菜蛾子房中表达。GO注释表明，多细胞生物生殖途径和多细胞生物生殖过程，以及配子发生和绒毛膜都得到了显著丰富。最可能与生殖相关的过程包括剪接体、泛素介导的蛋白水解、内吞作用、PI 3 K-Akt信号通路、胰岛素信号通路、cAMP信号通路和粘着斑，这些在前20个“高度代表”的KEGG通路中被鉴定。通过qRT-PCR进一步分析和验证了与卵子发生相关的功能基因，揭示了它们在小菜蛾生殖过程中的潜在主导作用。结论：我们新开发的小菜蛾卵巢转录组为该组织的基因表达谱以及与卵巢发育和卵子发生密切相关的功能基因网络提供了一个概述。这是第一个包含功能激活基因子集的小菜蛾卵巢全基因组转录组数据集。该方法为进一步研究小菜蛾繁殖的分子机制、筛选潜在的害虫综合治理分子靶标奠定了基础。
Background: As a specialized organ, the insect ovary performs valuable functions by ensuring fecundity and population survival. Oogenesis is the complex physiological process resulting in the production of mature eggs, which are involved in epigenetic programming, germ cell behavior, cell cycle regulation, etc. Identification of the genes involved in ovary development and oogenesis is critical to better understand the reproductive biology and screening for the potential molecular targets in Plutella xylostella, a worldwide destructive pest of economically major crops.Results: Based on transcriptome sequencing, a total of 7.88 Gb clean nucleotides was obtained, with 19,934 genes and 1861 new transcripts being identified. Expression profiling indicated that 61.7% of the genes were expressed (FPKM 1) in the P. xylostella ovary. GO annotation showed that the pathways of multicellular organism reproduction and multicellular organism reproduction process, as well as gamete generation and chorion were significantly enriched. Processes that were most likely relevant to reproduction included the spliceosome, ubiquitin mediated proteolysis, endocytosis, PI3K-Akt signaling pathway, insulin signaling pathway, cAMP signaling pathway, and focal adhesion were identified in the top 20 'highly represented' KEGG pathways. Functional genes involved in oogenesis were further analyzed and validated by qRT-PCR to show their potential predominant roles in P. xylostella reproduction.Conclusions: Our newly developed P. xylostella ovary transcriptome provides an overview of the gene expression profiling in this specialized tissue and the functional gene network closely related to the ovary development and oogenesis. This is the first genome-wide transcriptome dataset of P. xylostella ovary that includes a subset of functionally activated genes. This global approach will be the basis for further studies on molecular mechanisms of P. xylostella reproduction aimed at screening potential molecular targets for integrated pest management.