The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity
The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity
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DOI：
10.1016/j.febslet.2005.09.058
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发表时间：
2005-10-24
期刊：
影响因子：
3.5
通讯作者：
Sanders, D
中科院分区：
文献类型：
--
作者：
Peiter, E;Fischer, M;Sanders, D
Cch1p和Mid1p是酵母细胞质膜上高亲和力的钙离子通透通道的组成部分。在这里，我们表明突变体在Cch1pMid1p通道中的生长对低温和介质中的高铁浓度明显敏感。高浓度的Ca~(2+)对两种表型均有抑制作用。铁胁迫引起野生型和CC1 Delta Mid Delta酵母细胞内钙离子内流增加。抑制钙调神经磷酸酶强烈抑制铁胁迫下野生型酵母的生长，表明钙调神经磷酸酶是铁胁迫反应的下游元件。Cch1 Delta MID1 Delta突变体的铁过敏性与铁摄取增加无关。研究发现，抗氧化剂生育酚乙酸酯和(乙基)谷胱甘肽改善了铁胁迫突变体的生长和生存能力，表明氧化应激参与了铁敏感表型。此外，在铁存在的情况下，谷胱甘肽的氧化程度增加。结果表明，铁胁迫导致氧化平衡增加，Cch1pMid1p是耐受这种情况所必需的。(C)2005年，由Elsevier B.V.代表欧洲生化学会联合会出版。
Cch1p and Mid1p are components of a high-affinity Ca2+-permeable channel in the yeast plasma membrane. Here, we show that growth of mutants in the Cch1pMid1p channel is markedly hypersensitive to low temperature and to high iron concentration in the medium. Both phenotypes were suppressed by high Ca2+ concentration. Iron stress elicited an increased Ca2+ influx into both wild type and cch1 Delta mid Delta yeast. Inhibition of calcineurin strongly depressed growth of iron-stressed wild type yeast, indicating that calcineurin is a downstream element of the iron stress response. Iron hypersensitivity of the cch1 Delta mid1 Delta mutant was not associated with an increased iron uptake. An involvement of oxidative stress in the iron-hypersensitive phenotype was indicated by the findings that the antioxidants tocopheryl acetate and (ethyl)glutathione improved growth and viability of the iron-stressed mutant. Further, the degree of glutathione oxidation was increased in the presence of iron. The results indicate that iron stress leads to an increased oxidative poise and that Cch1pMid1p is essential to tolerate this condition. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.