A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers
A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers
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DOI：
10.1007/s00122-002-1189-5
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发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Baldoni, L
中科院分区：
文献类型：
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作者：
de la Rosa, R;Angiolillo, A;Baldoni, L
首次绘制了橄榄(Olea Europaea L.)以随机扩增多态DNA(RAPD)和扩增片段长度多态(AFLP)为显性标记，少数限制性片段长度多态(RFLP)和简单序列重复序列(SSR)为共显性标记构建基因组。利用Leccino和Dolce Agogia两个高度杂合的橄榄品种杂交后代的95个单株，采用伪测交策略。从61个随机引物中，共扩增出279个标记，其中Leccino标记158个，Dolce Agogia标记121个。21对AFLP引物组合共扩增出304个有用标记，其中Leccino杂合子160个，Dolce Agogia杂合子144个。在Leccino图谱中，共连锁了249个标记，其中包括110个随机扩增标记、127个AFLP标记、8个RFLP标记和3个SSR标记。这导致22个主连锁群和17个小连锁群的标记少于4个。在Dolce Agogia图谱中，共连锁了236个标记，其中93个是随机扩增标记，133个是AFLP标记，6个是RFLP标记，4个是SSR标记，共获得27个主连锁群和3个小连锁群。利用共显性RFLP和SSR，以及异源双链构型中的少数RAPD，建立了亲本连锁群之间的同源性。在Leccino和Dolce Agogia地图上，覆盖的总距离分别为2765厘米和2445厘米。Leccino和Dolce Agogia的平均标记间距分别为13.2 cM和11.9 cM。AFLP和RAPD标记在所报道的所有连锁群中均为均匀分布。将硬脂酰ACP去饱和酶基因定位于cv的第4连锁群。莱奇诺。
The first linkage map of the olive (Olea europaea L.) genome has been constructed using random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphisms (AFLP) as dominant markers and a few restriction fragment length polymorphisms (RFLP) and simple-sequence repeats (SSR) as codominant markers. Ninety-five individuals of a cross progeny derived from two highly heterozygous olive cultivars, Leccino and Dolce Agogia, were used by applying the pseudo test-cross strategy. From 61 RAPD primers 279 markers were obtained - 158 were scored for Leccino and 121 for Dolce Agogia. Twenty-one AFLP primer combinations gave 304 useful markers - 160 heterozygous in Leccino and 144 heterozygous in Dolce Agogia. In the Leccino map 249 markers (110 RAPD, 127 AFLP, 8 RFLP and 3 SSR) were linked. This resulted in 22 major linkage groups and 17 minor groups with fewer than four markers. In the Dolce Agogia map, 236 markers (93 RAPD, 133 AFLP, 6 RFLP and 4 SSR) were linked; 27 major linkage groups and three minor groups were obtained. Codominant RFLPs and SSRs, as well as few RAPDs in heteroduplex configuration, were used to establish homologies between linkage groups of both parents. The total distance covered was 2,765 cM and 2,445 cM in the Leccino and Dolce Agogia maps, respectively. The mean map distance between adjacent markers was 13.2 cM in Leccino and 11.9 cM in Dolce Agogia, respectively. Both AFLP and RAPD markers were homogeneously distributed in all of the linkage groups reported. The stearoyl-ACP desaturase gene was mapped on linkage group 4 of cv. Leccino.