Isolation of a strain of Acidithiobacillus caldus and its role in bioleaching of chalcopyrite
Isolation of a strain of Acidithiobacillus caldus and its role in bioleaching of chalcopyrite
复制标题
DOI：
10.1007/s11274-007-9350-6
复制
发表时间：
2007-02
影响因子：
4.1
通讯作者：
Q. Zhou;Fu Bo;Zhou Hong Bo;L. Xi;G. Jian;Liu Fei Fei-Liu-Fei-Fei-138085066;Chen Xin Hua
中科院分区：
文献类型：
--
作者：
Q. Zhou;Fu Bo;Zhou Hong Bo;L. Xi;G. Jian;Liu Fei Fei-Liu-Fei-Fei-138085066;Chen Xin Hua
从煤堆排水中分离到一株中度嗜热嗜酸的硫氧化细菌S2。该细菌是能动的，革兰氏阴性，杆状，测量为0.4至0.6乘1至2 μm，并且在42-45°C和2.5的初始pH下生长最佳。菌株S2以单质硫、硫代硫酸钠和连四硫酸钾为能源进行自养生长。该菌株不利用有机物和硫酸亚铁、黄铁矿、黄铜矿等无机矿物作为能源。通过形态、生理生化特征及16 S rRNA基因序列分析，表明菌株S2与喜温嗜酸硫杆菌（Acidithiobacillus caldus）的亲缘关系最近（基因序列相似性>99%）。将S2菌株与嗜铁小球藻或嗜酸氧化亚铁硫杆菌联合应用于黄铜矿生物浸出，提高了铜的浸出率。扫描电子显微镜（SEM）分析表明，黄铜矿表面在嗜铁细杆菌和酸性热硫杆菌的混合培养严重蚀刻。能量色散X射线（EDX）分析表明，嗜酸热硫杆菌（Acidithiobacilluscaldus）可通过氧化浸出过程中生成的硫来提高黄铜矿中铜的回收率。
A moderately thermophilic and acidophilic sulfur-oxidizing bacterium named S2, was isolated from coal heap drainage. The bacterium was motile, Gram-negative, rod-shaped, measured 0.4 to 0.6 by 1 to 2 μm, and grew optimally at 42–45°C and an initial pH of 2.5. The strain S2grew autotrophically by using elemental sulfur, sodium thiosulfate and potassium tetrathionate as energy sources. The strain did not use organic matter and inorganic minerals including ferrous sulfate, pyrite and chalcopyrite as energy sources. The morphological, biochemical, physiological characterization and analysis based on 16S rRNA gene sequence indicated that the strain S2is most closely related toAcidithiobacillus caldus(>99% similarity in gene sequence). The combination of the strain S2withLeptospirillum ferriphilumorAcidithiobacillus ferrooxidansin chalcopyrite bioleaching improved the copper-leaching efficiency. Scanning electron microscope (SEM) analysis revealed that the chalcopyrite surface in a mixed culture ofLeptospirillum ferriphilumandAcidithiobacillus calduswas heavily etched. The energy dispersive X-ray (EDX) analysis indicated thatAcidithiobacillus caldushas the potential role to enhance the recovery of copper from chalcopyrite by oxidizing the sulfur formed during the bioleaching progress.