The quantitative and condition-dependent Escherichia coli proteome.
The quantitative and condition-dependent Escherichia coli proteome.
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DOI：
10.1038/nbt.3418
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发表时间：
2016-01
影响因子：
46.9
通讯作者：
Heinemann M
中科院分区：
文献类型：
--
作者：
Schmidt A;Kochanowski K;Vedelaar S;Ahrné E;Volkmer B;Callipo L;Knoops K;Bauer M;Aebersold R;Heinemann M
测量蛋白质的精确浓度可以深入了解生物过程。在这里，我们使用有效的蛋白质提取和样品分馏和最先进的定量质谱技术，以产生一个全面的，条件依赖的蛋白质丰度图大肠杆菌。我们测量了55%的预测E.大肠杆菌基因（>2300蛋白质）在22种不同的实验条件下，并确定甲基化和N-末端蛋白质乙酰化以前不知道是普遍存在于细菌。我们揭示了全系统的蛋白质组分配，表达调控和翻译后适应。这些数据为系统生物学和更广泛的E.大肠杆菌研究社区。
Measuring precise concentrations of proteins can provide insights into biological processes. Here, we use efficient protein extraction and sample fractionation and state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein abundance map of Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation, and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.