Detection of Four Germ Cell Markers in Rats during Testis Morphogenesis: Differences and Similarities with Mice
Detection of Four Germ Cell Markers in Rats during Testis Morphogenesis: Differences and Similarities with Mice
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DOI：
10.1159/000329245
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Stumpp, Taiza
中科院分区：
文献类型：
--
作者：
Encinas, Giselly;Zogbi, Camila;Stumpp, Taiza
生殖细胞是唯一能够将遗传信息代代相传的细胞。生殖细胞发育在不同物种中得到了广泛的研究。在哺乳动物中，小鼠是大多数生殖细胞分化、性别决定和遗传学研究中使用的模型。在本研究中，我们认为，大鼠也是一个非常重要的模型研究生殖细胞发育的机制。为了研究大鼠生殖细胞的发育，并与小鼠生殖细胞的发育进行比较，对大鼠生殖细胞核抗原1（GCNA 1）、OCT 4、小鼠血管同源物（MVH）和特异性表面胚胎抗原1（SSEA 1）在胚胎和生后发育的不同阶段进行了免疫标记。SSEA1和GCNA1在大鼠原始生殖细胞和胚胎生殖细胞中均未检测到。GCNA1在生后被检测到，并且仅存在于细线期、偶线期和粗线期早期的精母细胞中。另一方面，在小鼠中，这些标记物在交配后11.5天（dpc）就在生殖细胞中被检测到。在迁移的大鼠原始生殖细胞以及已经定植于性腺的原始生殖细胞中检测到MVH，而在小鼠中，仅在已经到达性腺的生殖细胞中检测到MVH。在大鼠中，OCT 4阳性生殖细胞检测到13至17 dpc，但不是在19 dpc或出生后的睾丸。这与在胚胎和成年睾丸中显示OCT 4标记的小鼠形成对比。这些数据表明，大鼠和小鼠的原始生殖细胞发育显示出相当大的差异，大鼠也可能是研究生殖细胞胚胎发育的重要模型。版权所有（C）2011 S. Karger AG，巴塞尔
Germ cells are the only cells capable of transmitting genetic information from generation to generation. Germ cell development has been widely studied in different species. Among mammals, the mouse is the model used in the majority of studies on germ cell differentiation, sex determination and genetics. In the present study, we suggest that the rat is also a very important model for the investigation of the mechanisms of germ cell development. To study rat germ cell development and compare it with that of mouse, the germ cell markers germ cell nuclear antigen 1 (GCNA1), OCT4, mouse vasa homologue (MVH) and specific surface embryonic antigen 1 (SSEA1) were immunolabeled at different phases of embryonic and postnatal development. SSEA1 and GCNA1 were not detected in rat primordial germ cells and fetal gonocytes. GCNA1 was detected postnatally and was present only in leptotene, zygotene and early pachytene spermatocytes. On the other hand, in mice, these markers were detected in germ cells as soon as 11.5 days postcoitum (dpc). MVH was detected in migrating rat primordial germ cells as well as in those that have already colonized the gonads, whereas in mice, MVH is detected only in germ cells that have reached the gonads. In rats, OCT4-positive germ cells were detected from 13 to 17 dpc, but not at 19 dpc or in postnatal testes. This is in contrast with mice that show OCT4 labeling in both embryonic and adult testes. These data suggest that primordial germ cell development in rats and mice shows considerable differences and that the rat may also be an important model to study the embryonic development of germ cells. Copyright (C) 2011 S. Karger AG, Basel