ETHYLENE INSENSITIVE 3 suppresses plant de novo root regeneration from leaf explants and mediates age-regulated regeneration decline
ETHYLENE INSENSITIVE 3 suppresses plant de novo root regeneration from leaf explants and mediates age-regulated regeneration decline
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ETHLENE INSENSITIVE 3 抑制叶外植体的植物从头再生根并介导年龄调节的再生衰退
DOI：
10.1242/dev.179457
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发表时间：
2020-05-01
期刊：
影响因子：
4.6
通讯作者：
Zhu, Ziqiang
中科院分区：
文献类型：
--
作者：
Li, Hong;Yao, Lulu;Zhu, Ziqiang
强大的再生能力使植物在受伤时能够存活。例如，即使在没有任何外源植物激素处理的情况下，不定根也可以从分离的拟南芥叶外植体中的切割位点再生。这个过程被称为从头根再生（DNRR）。虽然DNRR的发育程序是已知的，但DNRR的精确调控机制还不完全清楚。在这里，我们表明，乙烯处理或转录因子乙烯不敏感3（EIN 3）的遗传激活强烈抑制DNRR率，而缺乏EIN 3及其同源物EIL 1（ein 3 eil 1）的突变体显示更高的DNRR能力。先前的报道表明，顺序诱导WUSCHEL相关的HOMEOBOX 11（WOX 11）/WOX 12和WOX 5/WOX 7表达是DNRR建立所必需的。我们发现EIN 3直接靶向WOX 11和WOX 5启动子区域以抑制它们的转录。此外，较老的植物表现出增强的EIN 3活性，并抑制WOX 11和WOX 5的表达。综上所述，这些结果说明植物衰老至少部分地利用EIN 3作为负调节因子通过抑制WOX基因的激活来抑制DNRR。
Powerful regeneration ability enables plant survival when plants are wounded. For example, adventitious roots can regenerate from the cutting site in detached Arabidopsis thaliana leaf explants, even in the absence of any exogenous plant hormone treatment. This process is known as de novo root regeneration (DNRR). Although the developmental program underlying DNRR is known, the precise regulatory mechanisms underlying DNRR are not completely understood. Here, we show that ethylene treatment or genetic activation of transcription factor ETHYLENE INSENSITIVE 3 (EIN3) strongly suppresses DNRR rates, while a mutant lacking EIN3 and its homolog EIL1 (ein3 eil1) displays a higher DNRR capacity. Previous reports have shown that the sequential induction of WUSCHEL RELATED HOMEOBOX 11 (WOX11)/WOX12 and WOX5/WOX7 expression is required for the establishment of DNRR. We found that EIN3 directly targets WOX11 and WOX5 promoter regions to suppress their transcription. Furthermore, older plants show enhanced EIN3 activity, and repressed expression of WOX11 and WOX5. Taken together, these results illustrate that plant aging at least partially takes advantage of EIN3 as a negative regulator to suppress DNRR through inhibiting the activation of WOX genes.