Class-selective collisionally activated dissociation/ion-molecule reactions of 4-quinolone antibiotics.
Class-selective collisionally activated dissociation/ion-molecule reactions of 4-quinolone antibiotics.
复制标题
4-喹诺酮抗生素的类选择性碰撞激活解离/离子分子反应。
DOI：
10.1021/ac00086a019
复制
发表时间：
1994
影响因子：
7.4
通讯作者：
Brodbelt,J
中科院分区：
文献类型：
--
作者：
Colorado,A;Brodbelt,J
在四极杆离子阱质谱仪中，建立了一种新的碰撞活化解离和离子-分子反应相结合的方法，用于对一类重要的抗生素4-醌类抗生素的类别选择性测定。在该方法中，碰撞活化解离被用来促进每个质子化的奎诺克碱脱水，并且所产生的脱水碎片离子与辅助试剂进行加成反应。包括丙酮、乙酸酐和正丙胺的辅助试剂具有特别允许与分析物离子形成环状加合物的结构。这种连续的过程是一类分析物分子的特征，这类分析物分子具有相邻的羧酸和酮官能团，并在刚性环上进行简单有效的脱水。随着分析问题的日益复杂和多样性，串联质谱的应用在过去十年中有了巨大的增长。化学电离（Cl）1 2和碰撞活化解离（CAD）3 456789通常用作互补技术，其中Cl用于使分析物质子化或产生新的加合物，CAD用于提供确证性结构鉴定。使用离子-分子反应选择性地衍生化气相中的分子也被证明是特别有用的辅助结构特异性工具。4-9在许多类型的分析中，主要目标是开发
A new combination of collisionallyactivated dissociation and ion-molecule reactions in a quadrupole ion trap mass spec-trometer isdeveloped for the class-selective determination of4-quinokmes, an important group of antibiotics. In thismethod, collisionally activated dissociation is used to promote dehydra-tion of each protonated quinokme, and the resulting dehydrated fragment ion undergoes an addition reaction with an auxiliary reagent. The auxiliary reagents, including acetone, acetic anhydride, and n-propylamine, have structures that specifically allow formation of a cyclic adduct with the analyte ion. This sequential process is characteristic of a class of analyte molecules that have adjacent carboxylic acid and ketone functional groupsanchored on a rigid ring and undergo facile, efficient dehydration.Applications of tandem mass spectrometry1 have grown tremendously over the past decade with the increasing complexity and variety of analytical problems. Chemical ionization (Cl) 1 2 and collisionally activated dissociation (CAD) 3 456789are typically used as complementary techniques, in which Cl is used to protonate the analyte or generate new adducts, with CAD used to provide confirmatorystructural identification. The use of ion-molecule reactionsto selectively derivatize molecules in the gas phase has also proved to be particularly useful as an auxiliary structurally specific tool. 4-9 In many types of analysis, the primary objective is to develop