How specific is CRISPR/Cas9 really?
How specific is CRISPR/Cas9 really?
复制标题
DOI：
10.1016/j.cbpa.2015.10.001
复制
发表时间：
2015-12
影响因子：
7.8
通讯作者：
Segal DJ
中科院分区：
文献类型：
--
作者：
O'Geen H;Yu AS;Segal DJ
随着RNA引导的核酸酶在基础研究和治疗开发中的广泛应用，其特异性引起了人们的极大兴趣。有关动物模型的简单生成和细胞基因组工程的报道引发了对靶向准确性的质疑。相互矛盾的早期报告使该领域认为CRISPR/CAS9系统是混杂的，导致了各种提高特异性的策略和越来越敏感的方法来检测偏离目标的事件。然而，其他研究表明，CRISPR/Cas9是一种高度特异的基因组编辑工具。这篇评论将集中于破译和解释这些看似对立的说法。
The specificity of RNA-guided nucleases has gathered considerable interest as they become broadly applied to basic research and therapeutic development. Reports of the simple generation of animal models and genome engineering of cells raised questions about targeting precision. Conflicting early reports led the field to believe that CRISPR/Cas9 system was promiscuous, leading to a variety of strategies for improving specificity and increasingly sensitive methods to detect off-target events. However, other studies have suggested that CRISPR/Cas9 is a highly specific genome-editing tool. This review will focus on deciphering and interpreting these seemingly opposing claims.