Properties of synthetic spider silk fibers based on Argiope aurantia MaSp2
Properties of synthetic spider silk fibers based on Argiope aurantia MaSp2
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DOI：
10.1021/bm701124p
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发表时间：
2008-06-01
影响因子：
6.2
通讯作者：
Lewis, Randolph V.
中科院分区：
文献类型：
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作者：
Brooks, Amanda E.;Stricker, Shane M.;Lewis, Randolph V.
蜘蛛进化出了一套复杂的吐丝腺体系统。每一个腺体都产生具有强度和弹性的丝，以适应其生物学目的。大壶腹丝的序列分析揭示了四个高度保守的串联氨基酸块：（GA）（n）、A（n）、GPGXX和GGX。虽然GPGXX基序被假设为负责纤维的延展性，但其精确的序列排列和含量显示出自然变异，将这些差异与特定的纤维性质相关联是困难的。基于橙色金蛛序列的三个遗传构建体被工程化以在被另一个基序中断之前逐渐增加头-尾组装中的GPGXX重复的数量。合成蜘蛛丝纺丝原液的圆二色性和傅里叶变换红外光谱显示二级结构，其对应于GPGXX区域的重复数的增加和合成纺重组纤维的可延伸性的增加。
Spiders have evolved a complex system of silk producing glands. Each of the glands produces silk with strength and elasticity tailored to its biological purpose. Sequence analysis of the major ampullate silk reveals four highly conserved concatenated blocks of amino acids: (GA)(n), A(n), GPGXX, and GGX While the GPGXX motif, which has been hypothesized to be responsible for the extensibility of the fiber, displays natural variation in its precise sequence arrangement and content, correlating these differences with particular fiber properties has been difficult. Three genetic constructs based on the Argiope aurantia sequence were engineered to progressively increase the number of GPGXX repeats in a head-to-tail assembly prior to interruption by another motif. Circular dichroism and Fourier transform infrared spectroscopy of synthetic spider silk spin dopes show secondary structures that correspond to an increase in the repeat number of GPGXX regions and an increase in the extensibility of synthetically spun recombinant fibers.