Regulation of the biopotency of primate luteinizing hormone by gonadotropin-releasing hormone in vitro and in vivo.
Regulation of the biopotency of primate luteinizing hormone by gonadotropin-releasing hormone in vitro and in vivo.
复制标题
体外和体内促性腺激素释放激素对灵长类黄体生成激素生物效能的调节。
DOI：
10.1095/biolreprod43.6.1045
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发表时间：
1990
影响因子：
3.6
通讯作者：
Robinson,JA
中科院分区：
文献类型：
--
作者：
Matteri,RL;Dierschke,DJ;Bridson,WE;Rhutasel,NS;Robinson,JA
Gonadotropin biological/immunological (B/I) ratios have proven to be valuable indicators of the biopotencies of LH and FSH. Observations of rapidly changing LH B/I have been made which suggest the existence of a readily mobilized pool of highly bioactive pituitary gonadotropins. To test this hypothesis, we have examined the role of GnRH in the regulation of LH B/I in vivo and in vitro. The rhesus monkey was used as a model due to its many physiological similarities with the human. A rapid elevation in circulating LH B/I was observed following GnRH administration to male monkeys that was sustained for at least 2 h (15 min;p< 0.05). The administration of 1 or 10 nM GnRH to cultured pituitary cells was found to significantly increase the B/I of secreted, but not intracellular, LH (p< 0.05). In unstimulated controls, the B/I of intracellular LH was higher than that of secreted LH (p< 0.05). These findings are consistent with the notion that a pool of highly active LH exists within the gonadotrophs in primates. One way that GnRH may regulate the bioactivity of circulating LH is by rapidly mobilizing this gonadotropin pool.
