Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus
Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus
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转录组分析揭示了冬虫夏草无性型菌株腺苷生物合成的分子机制
DOI：
10.1155/2019/1864168
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发表时间：
2019-12-11
影响因子：
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通讯作者：
Cai, Zhiming
中科院分区：
文献类型：
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作者：
Lin, Shan;Zou, Zhicheng;Cai, Zhiming
冬虫夏草是一种著名的真菌中药。为了揭示腺苷生物合成早期和晚期的分子变化，对变型真菌进行了转录组分析。共鉴定出2764个差异表达基因（DEGs） （p≤0.05,|log2 Ratio|≥1），其中上调1737个，下调1027个。4-10 d的基因表达谱显示嘌呤代谢途径的表达发生了明显的变化。通过qPCR验证了17个与嘌呤代谢相关的deg （map00230）的差异表达，表达趋势与RNA-Seq结果一致。随后，结合qPCR预测的腺苷生物合成途径和RNA-Seq基因表达数据表明，腺苷积累增加是ndk、ADK和APRT基因下调结合AK基因上调的结果。本研究对了解虫草中腺苷生物合成的分子机制具有重要意义。
Caterpillar fungus is a well-known fungal Chinese medicine. To reveal molecular changes during early and late stages of adenosine biosynthesis, transcriptome analysis was performed with the anamorph strain of caterpillar fungus. A total of 2,764 differentially expressed genes (DEGs) were identified (p ≤ 0.05, |log2 Ratio| ≥ 1), of which 1,737 were up-regulated and 1,027 were down-regulated. Gene expression profiling on 4–10 d revealed a distinct shift in expression of the purine metabolism pathway. Differential expression of 17 selected DEGs which involved in purine metabolism (map00230) were validated by qPCR, and the expression trends were consistent with the RNA-Seq results. Subsequently, the predicted adenosine biosynthesis pathway combined with qPCR and gene expression data of RNA-Seq indicated that the increased adenosine accumulation is a result of down-regulation of ndk, ADK, and APRT genes combined with up-regulation of AK gene. This study will be valuable for understanding the molecular mechanisms of the adenosine biosynthesis in caterpillar fungus.