Enzyme kinetic studies from progress curves.
Enzyme kinetic studies from progress curves.
复制标题
从进度曲线进行酶动力学研究。
作者：
E. Canela;R. Franco
描述了一种方法，用于拟合从进展曲线获得的速度的稳态速率方程。它基于Markus和Plesser [（1981）in Kinetic Data Analysis：Design and Analysis of Enzyme and Kinetic Data（Edrenyi，ed.），pp. 317-339，Plenum Press，纽约]。需要动力学参数的良好初始估计的障碍通过使用通过Yun和Suelter的方法的微小修改以图形方式提供的参数来消除[（1977）Biochim，Biophys. Acta 480，1-13]。这种基于进度曲线的方法可以像基于初始速度的方法一样在竞争模型之间进行相同的区分，从而大大节省了实验时间。BASIC和FORTRAN 77程序作为补充出版物1050132（17页）存放在英国西约克郡LS 23 7 BQ，Wetherby，Boston Spa，British Library（Lending Division），可根据Biochem. J.（1986）233，5-6中所示的条件从其获得拷贝。
A method is described for fitting the velocities obtained from progress curves to a steady-state rate equation. It is based on the method of Markus & Plesser [(1981) in Kinetic Data Analysis: Design and Analysis of Enzyme and Kinetic Data (Edrenyi, ed.), pp. 317-339, Plenum Press, New York]. The obstacle of needing good initial estimates of kinetic parameters is removed by using the parameters provided graphically by a minor modification of the method of Yun & Suelter [(1977) Biochim, Biophys. Acta 480, 1-13]. This progress-curved-based method allows the same discrimination among rival models as do the initial-velocity-based methods, with a great saving of experimental time. The BASIC and FORTRAN 77 programs are deposited as Supplementary Publication SUP 50132 (17 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1986) 233, 5-6.