Correlation between osteopontin and caveolin-1 in the pathogenesis and progression of osteoarthritis
Correlation between osteopontin and caveolin-1 in the pathogenesis and progression of osteoarthritis
复制标题
骨桥蛋白和caveolin-1在骨关节炎发病机制和进展中的相关性
DOI：
10.3892/etm.2015.2433
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发表时间：
2015-06-01
影响因子：
2.7
通讯作者：
Hua, Lei Guang
中科院分区：
文献类型：
--
作者：
Min, Tu;Sheng, Li Yu;Hua, Lei Guang
关于骨桥蛋白（OPN）和小窝蛋白-1在骨关节炎（OA）病理学中的作用，先前的研究产生了相互矛盾的结果。因此，本研究的目的是探讨骨桥蛋白和小窝蛋白-1在OA发病机制和进展中的相关性。从50个个体获得软骨组织样品，其中40个被诊断患有OA，10个是正常健康个体。根据改良的Mankin分级系统将样本分为正常、轻度、中度和重度四组。免疫组化法检测骨桥蛋白（OPN）和小窝蛋白-1（caveolin-1）的表达。OPN和caveolin-1在所有四组的组织中均被检测到。各组间OPN表达水平相互比较差异有统计学意义（P< 0.05）。除中、重度组间差异无统计学意义（P> 0.05）外，其余各组间差异均有统计学意义（P <0.05）。改良Mankin评分与各组OPN表达的平均灰度值（r=-0.824，P< 0.01）和caveolin-1表达的平均灰度值（r= 0.725，P< 0.01）相关。骨桥蛋白的平均灰度值与小窝蛋白-1的表达呈负相关（r=-0.676，P.0.05）。因此，本研究的结果表明，骨桥蛋白和小窝蛋白-1之间的相关性可能在OA的发病机制和进展中发挥重要作用。
Previous studies have produced contradictory results with regard to the role of osteopontin (OPN) and caveolin- 1 in the pathology of osteoarthritis (OA). Thus, the aim of the present study was to investigate the correlation between OPN and caveolin-1 in the pathogenesis and progression of OA. Cartilage tissue samples were obtained from 50 individuals, of which 40 had been diagnosed with OA and 10 were normal healthy individuals. The samples were ascribed to four groups, namely the normal, minor, moderate and severe groups, on the basis of the improved Mankin grading system. Immunohistochemistry was applied to analyse the expression of OPN and caveolin-1. OPN and caveolin-1 were detected in the tissues of all four groups. The mutual comparisons of OPN expression levels among the groups revealed statistically significant differences (P< 0.05). In addition, the mutual comparisons of caveolin-1 expression levels among the four groups demonstrated statistically significant differences (P< 0.05), with the exception of that between the moderate and severe groups (P> 0.05). Improved Mankin grading system scores were shown to correlate with the average grey level of OPN expression in each group (r= -0.824, P< 0.01) and the average grey level of caveolin-1 expression (r= 0.725, P< 0.01). Furthermore, a statistically significant negative correlation was observed between the average grey levels of OPN and caveolin-1 expression (r= -0.676, P. 0.05). Therefore, the results of the present study indicated that the correlation between OPN and caveolin-1 may play a significant role in the pathogenesis and progression of OA.