Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor
Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor
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DOI：
10.1021/ja060005h
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发表时间：
2006-02-22
影响因子：
15
通讯作者：
Collins, G
中科院分区：
文献类型：
--
作者：
Hansen, JA;Wang, J;Collins, G
第一次显示了适体与无机纳米晶体的编码和扩增特征的耦合，以提供对几种蛋白质靶标的高灵敏度和选择性的同时生物电子检测。这是在一个单步置换试验中完成的，该试验与几种巯基化适体的自组装单层有关，该适体与携带不同无机纳米晶体的蛋白质缀合。电化学剥离检测的非置换的核酸示踪剂的结果在一个非常低的（阿托摩尔）的检测限，即，显着低于现有的适体生物传感器。新设备为在疾病进展的早期阶段测量大量超低水平的疾病标志物提供了很大的希望。
The coupling of aptamers with the coding and amplification features of inorganic nanocrystals is shown for the first time to offer a highly sensitive and selective simultaneous bioelectronic detection of several protein targets. This is accomplished in a single-step displacement assay in connection to a self-assembled monolayer of several thiolated aptamers conjugated to proteins carrying different inorganic nanocrystals. Electrochemical stripping detection of the nondisplaced nanocrystal tracers results in a remarkably low (attomole) detection limit, that is, significantly lower than those of existing aptamer biosensors. The new device offers great promise for measuring a large panel of disease markers present at ultralow levels during early stages of the disease progress.