Herbivory induces a ROS burst and the release of volatile organic compounds in the fern Pteris vittata L.
Herbivory induces a ROS burst and the release of volatile organic compounds in the fern Pteris vittata L.
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DOI：
10.1080/17429140802387739
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Bossi, Simone
中科院分区：
文献类型：
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作者：
Imbiscuso, Gloria;Trotta, Antonio;Bossi, Simone
对砷超积累蕨类植物 Pteris vittata L. 进行了研究，以研究低等植物对机械 (MW) 和食草动物伤害 (HW) 的反应行为。为此，以棉叶虫 Spodoptera littoralis 为食 P. vittata，采用固相微萃取 (SPME) 检测蕨类植物顶部空间的挥发性有机化合物 (VOC) 排放量，并通过 GC-MS 进行分析。对照以未受伤的蕨类植物和MW蕨类植物为代表。荧光探针 Amplex red 用于通过共焦激光扫描显微镜研究 MW 和 HW 引起的氧化爆发。蕨类植物通过释放倍半萜(Z)-β-法呢烯、(E)-β-法呢烯、(Z,E)-α-法呢烯、(E,E)-α-法呢烯和(E)-橙花叔醇来应对食草动物受伤事件。在 MW 和 HW 蕨类植物的受伤区域附近都发现了氧化爆发，并且幼小的蕨类植物在 HW 后会产生更高的 H2O2 产量。报告的数据表明，低等植物，例如古代蕨类植物，在食草动物攻击后表现出在许多高等和更进化的植物中常见和报告的两种事件：氧化爆发和挥发性萜类化合物的排放。
The arsenic hyper-accumulating fern, Pteris vittata L., was studied to investigate the behavior of lower plants in response to both mechanical (MW) and herbivore wounding (HW). To this end, the cotton leaf worm Spodoptera littoralis was fed on P. vittata and the volatile organic compound (VOC) emission was detected from the fern's head space by using solid phase micro extraction (SPME) and analyzed by GC-MS. Controls were represented by unwounded and MW ferns. The fluorescent probe Amplex red was used to investigate the oxidative burst due to MW and HW by confocal laser scanning microscopy. Ferns responded to herbivore wounding events by emitting the sesquiterpenes (Z)-beta-farnesene, (E)-beta-farnesene, (Z, E)-alpha-farnesene, (E,E)-alpha-farnesene and (E)-nerolidol. An oxidative burst was found close to wounded areas in both MW and HW ferns, and young ferns reacted with a higher H2O2 production after HW. The reported data suggest that lower plants, such as ancient ferns, show two events commonly occurring and reported in many higher and more evolved plants after herbivore attack: an oxidative burst and the emission of volatile terpenoids.