Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo
Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo
复制标题
DOI：
10.1016/j.fertnstert.2009.10.044
复制
发表时间：
2010-10-01
影响因子：
6.7
通讯作者：
Macklon, Nick S.
中科院分区：
文献类型：
--
作者：
Boomsma, Carolien M.;Kavelaars, Annemieke;Macklon, Nick S.
目的：阐明卵巢刺激对子宫内环境的影响，以胚胎移植前抽取的子宫内膜分泌物中的细胞因子、趋化因子和生长因子谱为代表。设计：前瞻性队列研究。地点：三级转诊大学医院的生育中心。患者：招募了 42 名接受 GnRH 类似物卵巢刺激的患者。他们参加了自然周期和卵巢刺激周期，以进行患者内部比较。干预措施：在胚胎移植前立即进行子宫内膜分泌物抽吸。主要结果测量：通过多重免疫分析评估已知参与人类胚胎植入的 17 种介质的浓度。结果：经过多次测试校正后，白细胞介素 (IL)-1β、IL-5、IL-10、IL-12、IL-5、IL-10、IL-12 的浓度显着升高。与自然周期相比，刺激的子宫内膜分泌物中存在 IL-17、肿瘤坏死因子 (TNF)-α、肝素结合表皮生长因子 (HbEGF)、eotaxin 和 dickkopf 同源物-1。 结论：子宫内膜分泌物分析提供了一种研究卵巢刺激对子宫内环境影响的新方法。卵巢刺激显着改变胚胎移植后所遇到的体内环境。 （Fertil Steril (R) 2010；94:1764-8。(C)2010，美国生殖医学会。）
Objective: To elucidate the impact of ovarian stimulation on the intrauterine milieu represented by the cytokine, chemokine, and growth factor profile in endometrial secretions aspirated before embryo transfer.Design: Prospective cohort study.Setting: Fertility center in tertiary referral university hospital.Patient(s): Forty-two patients undergoing ovarian stimulation with GnRH analogues were recruited. They participated in both a natural and an ovarian-stimulated cycle for within patient comparisons.Intervention(s): Endometrial secretion aspiration was performed immediately before embryo transfer.Main Outcome Measure(s): The concentrations of 17 mediators known to be involved in human embryo implantation were assessed by multiplex immunoassay.Result(s): After correction for multiple testing, significantly higher concentrations of interleukin (IL)-1 beta, IL-5, IL-10, IL-12, IL-17, tumor necrosis factor (TNF)-alpha, heparin-binding epidermal growth factor (HbEGF), eotaxin, and dickkopf homologue-1 were present in endometrial secretions obtained in stimulated compared with natural cycles.Conclusion(s): Endometrial secretion analysis provides a novel means of investigating the effect of ovarian stimulation on the intrauterine milieu. The in vivo milieu encountered by the embryo after transfer is significantly altered by ovarian stimulation. (Fertil Steril (R) 2010;94:1764-8. (C)2010 by American Society for Reproductive Medicine.)