Chloroplast behaviour and interactions with other organelles in Arabidopsis thaliana pavement cells
Chloroplast behaviour and interactions with other organelles in Arabidopsis thaliana pavement cells
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DOI：
10.1242/jcs.202275
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发表时间：
2018-01-01
影响因子：
4
通讯作者：
Mathur, Jaideep
中科院分区：
文献类型：
--
作者：
Barton, Kiah A.;Wozny, Michael R.;Mathur, Jaideep
叶绿体是绿色植物的特征。叶肉细胞拥有大部分叶绿体，并且人们普遍认为，除了保卫细胞之外，大多数高等植物的表皮层不含叶绿体。然而，最近对拟南芥的观察表明，人行道细胞中的叶绿体群体比叶肉叶绿体小，并且具有高的基质与基粒比率。在这里，使用稳定的转基因株系表达针对质体基质，质膜，内质网，液泡膜，细胞核，线粒体，过氧化物酶体，F-肌动蛋白和微管的荧光蛋白，我们表征的时空之间的关系路面细胞叶绿体（PCC）和它们的亚细胞环境。对PCC的观察表明，表皮和叶肉层之间的源-汇关系，拟南芥突变体glabra 2（gl 2）和immutans（im），这表明改变了表皮质体发育的实验，强调了它们的发育可塑性。我们的研究结果奠定了基础，旨在了解PCCs在植物与环境相互作用中的确切作用和贡献的进一步调查。
Chloroplasts are a characteristic feature of green plants. Mesophyll cells possess the majority of chloroplasts and it is widely believed that, with the exception of guard cells, the epidermal layer in most higher plants does not contain chloroplasts. However, recent observations on Arabidopsis thaliana have shown a population of chloroplasts in pavement cells that are smaller than mesophyll chloroplasts and have a high stroma to grana ratio. Here, using stable transgenic lines expressing fluorescent proteins targeted to the plastid stroma, plasma membrane, endoplasmic reticulum, tonoplast, nucleus, mitochondria, peroxisomes, F-actin and microtubules, we characterize the spatiotemporal relationships between the pavement cell chloroplasts (PCCs) and their subcellular environment. Observations on the PCCs suggest a source-sink relationship between the epidermal and the mesophyll layers, and experiments with the Arabidopsis mutants glabra2 (gl2) and immutans (im), which show altered epidermal plastid development, underscored their developmental plasticity. Our findings lay down the foundation for further investigations aimed at understanding the precise role and contributions of PCCs in plant interactions with the environment.