The Pyrexia transient receptor potential channel mediates circadian clock synchronization to low temperature cycles in Drosophila melanogaster.
The Pyrexia transient receptor potential channel mediates circadian clock synchronization to low temperature cycles in Drosophila melanogaster.
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Pyrexia 瞬时受体电位通道介导果蝇生物钟与低温周期的同步。
DOI：
10.1098/rspb.2013.0959
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Wolfgang W
中科院分区：
文献类型：
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作者：
Wolfgang W
昼夜节律钟是一种内源性的24小时振荡器，在时间上调节许多生理和行为过程。为了对生物体有益，这些时钟必须每天与环境周期同步。光：暗和伴随的每日温度周期（TC）都起着Zeitgeber（“时间给予者”）的作用，并有效地引导生物钟。介导这种同步的温度受体尚未确定。瞬时受体电位（TRP）通道在动物体内起着温度感受器的作用，在这里我们证明了发热（Pyx）TRP通道介导了果蝇的温度同步化。Pyx在外周感觉器官（弦音器官）中表达，这些器官以前与温度同步有关。缺乏Pyx功能的果蝇在较低的温度范围（16-20°C）内无法使其行为与TC同步，这种缺陷可以通过引入Pyx基因的野生型拷贝来部分挽救。与较高TC的同步不受影响，表明Pyx在较低温度下的特定作用。此外，pyx突变体在暴露于TC后会加快他们的生物钟。我们的研究结果确定了第一个TRP通道参与温度同步的生物钟。
Circadian clocks are endogenous approximately 24 h oscillators that temporally regulate many physiological and behavioural processes. In order to be beneficial for the organism, these clocks must be synchronized with the environmental cycles on a daily basis. Both light : dark and the concomitant daily temperature cycles (TCs) function asZeitgeber(‘time giver’) and efficiently entrain circadian clocks. The temperature receptors mediating this synchronization have not been identified. Transient receptor potential (TRP) channels function as thermo-receptors in animals, and here we show that the Pyrexia (Pyx) TRP channel mediates temperature synchronization inDrosophila melanogaster. Pyx is expressed in peripheral sensory organs (chordotonal organs), which previously have been implicated in temperature synchronization. Flies deficient for Pyx function fail to synchronize their behaviour to TCs in the lower range (16–20°C), and this deficit can be partially rescued by introducing a wild-type copy of thepyxgene. Synchronization to higher TCs is not affected, demonstrating a specific role for Pyx at lower temperatures. In addition,pyxmutants speed up their clock after being exposed to TCs. Our results identify the first TRP channel involved in temperature synchronization of circadian clocks.