Novel perspective for protein-drug interaction analysis: atomic force microscope.
Novel perspective for protein-drug interaction analysis: atomic force microscope.
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蛋白质-药物相互作用分析的新视角：原子力显微镜。
DOI：
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Jian
中科院分区：
文献类型：
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作者：
Heng Sun;Jian
蛋白质是药物的主要靶点，药物-靶点相互作用的鉴定和分析是药物发现的重要因素。原子力显微镜（AFM）是一种强大的工具，使其能够成像蛋白质与纳米分辨率和探针分子间的力量在生理条件下。我们回顾了最近的研究领域进行的目标蛋白质药物发现使用基于AFM的分析技术，包括药物驱动的蛋白质形态和相互作用的纳米力学性质的变化。讨论了模型或活细胞表面药物-靶标相互作用和药物调节蛋白-蛋白相互作用（PPI）的基本机制（包括热力学和动力学参数）。此外，还讨论了该领域的挑战和前景。总的来说，这种对蛋白质-药物相互作用的机械性质的洞察为制药领域的合理药物发现提供了前所未有的信息框架。
Proteins are major drug targets, and drug-target interaction identification and analysis are important factors for drug discovery. Atomic force microscopy (AFM) is a powerful tool making it possible to image proteins with nanometric resolution and probe intermolecular forces under physiological conditions. We review recent studies conducted in the field of target protein drug discovery using AFM-based analysis technology, including drug-driven changes in nanomechanical properties of protein morphology and interactions. Underlying mechanisms (including thermodynamic and kinetic parameters) of the drug-target interaction and drug-modulating protein-protein interaction (PPI) on the surfaces of models or living cells are discussed. Furthermore, challenges and the outlook for the field are likewise discussed. Overall, this insight into the mechanical properties of protein-drug interactions provides an unprecedented information framework for rational drug discovery in the pharmaceutical field.