Physical nature of bacterial cytoplasm
Physical nature of bacterial cytoplasm
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DOI：
10.1103/physrevlett.96.098102
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发表时间：
2006-03-10
影响因子：
8.6
通讯作者：
Cox, EC
中科院分区：
文献类型：
--
作者：
Golding, I;Cox, EC
我们跟踪活的E. coli细胞。我们发现，该运动是subdiffusive的，具有对生理变化（包括细胞骨架元素的破坏）具有鲁棒性的指数。通过修改RNA分子和细菌细胞的参数，我们能够研究可能导致这种独特运动类型的可能机制，特别是大分子拥挤的影响。我们还研究了异常扩散对细菌基因调控动力学的影响，特别是转录因子如何找到它们的DNA靶点。
We track the motion of individual fluorescently labeled mRNA molecules inside live E. coli cells. We find that the motion is subdiffusive, with an exponent that is robust to physiological changes, including the disruption of cytoskeletal elements. By modifying the parameters of the RNA molecule and the bacterial cell, we are able to examine the possible mechanisms that can lead to this unique type of motion, especially the effect of macromolecular crowding. We also examine the implications of anomalous diffusion on the kinetics of bacterial gene regulation, in particular, how transcription factors find their DNA targets.