Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites
Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites
复制标题
DOI：
10.1126/science.1097468
复制
发表时间：
2004-06-18
期刊：
影响因子：
56.9
通讯作者：
Edwards, RH
中科院分区：
文献类型：
--
作者：
Fremeau, RT;Kam, K;Edwards, RH
囊泡谷氨酸转运体（VGLUTs）1和2在成体大脑中呈现相互排斥的分布，这表明其在具有不同释放特性的突触中具有特异性。与这种分布一致的是，VGLUT1基因失活使成体中一部分兴奋性神经元沉默。然而，相同的细胞群在生命早期表现出不依赖VGLUT1的传递。发育中的海马神经元在不同的突触位点短暂地共表达VGLUT2和VGLUT1，且具有不同的短期可塑性。VGLUT1的缺失也减少了突触小泡的储备池。因此，VGLUT1在神经末梢的膜转运中起着意想不到的作用。
Vesicular glutamate transporters (VGLUTs) 1 and 2 show a mutually exclusive distribution in the adult brain that suggests specialization for synapses with different properties of release. Consistent with this distribution, inactivation of the VGLUT1 gene silenced a subset of excitatory neurons in the adult. However, the same cell populations exhibited VGLUT1-independent transmission early in life. Developing hippocampal neurons transiently coexpressed VGLUT2 and VGLUT1 at distinct synaptic sites with different short-term plasticity. The loss of VGLUT1 also reduced the reserve pool of synaptic vesicles. Thus, VGLUT1 plays an unanticipated role in membrane trafficking at the nerve terminal.