Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses.
Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses.
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DOI：
10.1093/jxb/eru014
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发表时间：
2014-04
影响因子：
6.9
通讯作者：
Maeshima M
中科院分区：
文献类型：
--
作者：
Tanaka N;Kato M;Tomioka R;Kurata R;Fukao Y;Aoyama T;Maeshima M
拟南芥根毛对水分和几种矿物质的吸收、酸性磷酸酶和有机酸的分泌以及根的锚定具有重要作用。拟南芥的质膜相关钙结合蛋白-2参与了根毛尖的生长。在根毛特异的ExPANSIN A7启动子控制下过表达该蛋白的23个残基N-末端结构域的几个转基因品系完全缺乏根毛。在这些根无毛品系(NR23)中，研究了根毛在正常和胁迫条件下的作用。与野生型相比，NR23的吸水率降低了47%，抗旱性降低，适应高温的能力降低。在缺磷、缺铁、缺钙、缺锌、缺铜、缺钾的条件下，NR23的生长受到抑制。此外，在正常或缺乏特定矿物质的培养条件下，NR23的单个矿物质含量都较低。在野生型植物中，主根和侧根产生大量的根毛，在缺磷条件下这些根毛会变得拉长；NR23不产生根毛。尽管PHT1；1-PHT1；6等几种质膜磷酸转运蛋白的异构体在缺磷条件下生长后被显著诱导，但NR23的诱导水平不到野生型的一半。在缺磷条件下，NR23根分泌的酸性磷酸酶、苹果酸和柠檬酸分别是野生型根的38%、9%和16%。这些结果表明，根毛对水分和几种矿物质的吸收、酸性磷酸酶(S)和有机酸的分泌以及初级根向凝胶的渗透具有重要作用。
Root hairs of Arabidopsis play significant roles in the absorption of water and several minerals, secretion of acid phosphatases and organic acids, and anchoring of roots. The plasma membrane-associated Ca2+-binding protein-2 of Arabidopsis thaliana is involved in the growth of root hair tips. Several transgenic lines that overexpress the 23 residue N-terminal domain of this protein under the control of the root hair-specific EXPANSIN A7 promoter lack root hairs completely. The role of root hairs under normal and stress conditions was examined in one of these root hair-less lines (NR23). Compared with the wild type, NR23 showed a 47% reduction in water absorption, decreased drought tolerance, and a lower ability to adapt to heat. Growth of NR23 was suppressed in media deficient in phosphorus, iron, calcium, zinc, copper, or potassium. Also, the content of an individual mineral in NR23 grown in normal medium, or in medium lacking a specific mineral, was relatively low. In wild-type plants, the primary and lateral roots produce numerous root hairs that become elongated under phosphate-deficient conditions; NR23 did not produce root hairs. Although several isoforms of the plasma membrane phosphate transporters including PHT1;1–PHT1;6 were markedly induced after growth in phosphate-deficient medium, the levels induced in NR23 were less than half those observed in the wild type. In phosphate-deficient medium, the amounts of acid phosphatase, malate, and citrate secreted from NR23 roots were 38, 9, and 16% of the levels secreted from wild-type roots. The present results suggest that root hairs play significant roles in the absorption of water and several minerals, secretion of acid phosphatase(s) and organic acids, and in penetration of the primary roots into gels.
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影响因子：
4.5
作者：
Bruex A;Kainkaryam RM;Wieckowski Y;Kang YH;Bernhardt C;Xia Y;Zheng X;Wang JY;Lee MM;Benfey P;Woolf PJ;Schiefelbein J
通讯作者：
Schiefelbein J
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
影响因子：
7.3
作者：
Gahoonia, TS;Nielsen, NE
通讯作者：
Nielsen, NE
影响因子：
9.4
作者：
Chandrika, Nulu Naga Prafulla;Sundaravelpandian, Kalaipandian;Schmidt, Wolfgang
通讯作者：
Schmidt, Wolfgang
影响因子：
7.4
作者：
Gierth, M;Mäser, P;Schroeder, JI
通讯作者：
Schroeder, JI