Seamless tubular poly(glycerol sebacate) scaffolds: High-yield fabrication and potential applications
Seamless tubular poly(glycerol sebacate) scaffolds: High-yield fabrication and potential applications
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DOI：
10.1002/jbm.a.31598
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发表时间：
2008-08-01
影响因子：
4.9
通讯作者：
Wang, Yadong
中科院分区：
文献类型：
--
作者：
Crapo, Peter M.;Gao, Jin;Wang, Yadong
我们先前已经创建了由可生物降解的弹性体聚（癸二酸甘油酯）（PGS）组成的支架，其是管状的、无缝的和高度多孔的。在这里，我们描述了随后开发的两种支架制造方法，并通过检查整体产量和支架特性，如缺陷频率和严重程度，壁厚均匀性，微观结构，孔隙率和机械性能，将这些方法的优点与原始方法进行比较。用热收缩（HS）芯轴制造的支架具有更高的产量，更少的缺陷，更均匀的壁厚和微观结构，以及更高的孔隙率。屈服和支架特征的改善可能是由于力的分布更均匀，导致在取出HS芯轴期间支架中的应力集中更低。当在生物反应器中接种平滑肌细胞时，优化的支架保留了74%的细胞，这些细胞在体外培养21天后增殖并形成融合的细胞层。(C)2007 Wiley Periodicals，Inc.
We have previously created scaffolds composed of a biodegradable elastomer, poly(glycerol sebacate) (PGS), which are tubular, seamless, and highly porous. Here we describe two scaffold fabrication methods developed subsequently and compare the advantages of these methods to the original by examining overall yields and scaffold characteristics, such as defect frequency and severity, wall thickness homogeneity, microstructure, porosity, and mechanical properties. Scaffolds fabricated with a heat-shrinkable (HS) mandrel had higher yield, fewer defects, more homogeneous wall thickness and microstructure, and higher porosity. Improvements in yield and scaffold characteristics likely resulted from more uniform distribution of forces resulting in lower stress concentrations in the scaffolds during removal of HS mandrels. When seeded with smooth muscle cells in a bioreactor, the optimized scaffolds retained 74% of cells, which proliferated and formed a confluent cellular layer after 21 days of in vitro culture. (C) 2007 Wiley Periodicals, Inc.