Fluorescent proteins: a cell biologist's user guide.
Fluorescent proteins: a cell biologist's user guide.
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DOI：
10.1016/j.tcb.2009.08.002
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发表时间：
2009-11
影响因子：
19
通讯作者：
Snapp, Erik Lee
中科院分区：
文献类型：
--
作者：
Snapp, Erik Lee
荧光蛋白（FPs）已经彻底改变了细胞生物学。自克隆绿色荧光蛋白（GFP）以来，在活细胞中标记和可视化蛋白质的价值从成千上万的出版物中可见一斑。生物学家已经被丰富的FPs淹没了；然而，FP工具箱并没有必要为细胞生物学家进行优化。普通FP质粒对于FP融合蛋白的构建是次优的。更有问题的是，商业和研究者构建的fp融合蛋白会破坏重要的细胞靶向信息。即使当细胞生物学家正确构建FP融合蛋白时，也很少有不证自明的应该使用哪种FP。重要的FP信息，如低聚物形成或光稳定性，往往是无法搜索或轶事。这篇简短的指南是为了帮助正确地利用细胞中的FPs。
Fluorescent Proteins (FPs) have revolutionized cell biology. The value of labeling and visualizing proteins in living cells is evident from thousands of publications since the cloning of Green Fluorescent Protein (GFP). Biologists have been flooded with a cornucopia of FPs; however, the FP toolbox has not necessarily been optimized for cell biologists. Common FP plasmids are suboptimal for FP-fusion protein construction. More problematic are commercial and investigator-constructed FP-fusion proteins that disrupt important cellular targeting information. Even when cell biologists correctly construct FP-fusion proteins, it is rarely self-evident which FP should be used. Important FP information, such as oligomer formation or photostability, is often unsearchable or anecdotal. This brief guide is offered to assist in correctly exploiting FPs in cells.
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