Interaction of strain AD169 and a clinical isolate of cytomegalovirus with peripheral monocytes: the effect of lipopolysaccharide stimulation.
Interaction of strain AD169 and a clinical isolate of cytomegalovirus with peripheral monocytes: the effect of lipopolysaccharide stimulation.
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AD169 菌株和巨细胞病毒临床分离株与外周单核细胞的相互作用：脂多糖刺激的作用。
DOI：
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发表时间：
1987
影响因子：
6.4
通讯作者：
H. Garnett
中科院分区：
文献类型：
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作者：
L. Dudding;H. Garnett
通过使用实验室适应菌株 AD169 和最近的临床分离株，研究了人类巨细胞病毒 (CMV) 感染对来自血清阴性供体的脂多糖 (LPS) 刺激和未刺激的单核细胞的影响。感染该分离株的LPS刺激和未刺激的单核细胞均表现出早期CMV抗原的表达，并且与AD169感染的单核细胞相比，其对淋巴细胞增殖的抑制作用显着更强，后者很少表达可检测到的病毒蛋白。 LPS刺激和未刺激的单核细胞的人CMV感染导致白细胞介素1活性的消除，在感染CMV临床分离株的LPS刺激的单核细胞中效果显着。向受感染、受刺激的单核细胞中添加白细胞介素-1可完全恢复对植物血凝素的淋巴增殖反应，而向受感染、未受刺激的细胞中添加这种白细胞因子则不能恢复这种反应。
The effect of human cytomegalovirus (CMV) infection on lipopolysaccharide (LPS)-stimulated and unstimulated monocytes from seronegative donors was studied by using the laboratory-adapted strain AD169 and a recent clinical isolate. LPS-stimulated and unstimulated monocytes infected with the isolate showed expression of immediate-early CMV antigens and were significantly more suppressive for lymphocyte proliferation than were strain AD169-infected monocytes, which rarely expressed detectable viral protein. Human CMV infection of LPS-stimulated and unstimulated monocytes resulted in abrogation of interleukin-1 activity, with the effect being marked in LPS-stimulated monocytes infected with the clinical isolate of CMV. Addition of interleukin-1 to infected, stimulated monocytes completely restored lymphoproliferative responses to phytohemagglutinin, whereas addition of this leukokine to infected, unstimulated cells could not restore this response.