Effects of waterborne Cu exposure on intestinal copper transport and lipid metabolism of Synechogobius hasta
Effects of waterborne Cu exposure on intestinal copper transport and lipid metabolism of Synechogobius hasta
复制标题
水性铜暴露对聚鮈鱼肠道铜转运和脂质代谢的影响
DOI：
10.1016/j.aquatox.2016.08.001
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发表时间：
2016-09-01
影响因子：
4.5
通讯作者：
Pan, Ya-Xiong
中科院分区：
文献类型：
--
作者：
Chen, Feng;Luo, Zhi;Pan, Ya-Xiong
本研究旨在探讨水中铜暴露对水合虾（Synechogobius hasta）肠道铜转运和脂质代谢的影响。柽柳对M - Cu的暴露量分别为0、0.4721和0.9442亩。采样时间分别为第0、21和42天。分析生长性能、肠道脂质沉积、铜含量以及参与铜转运和脂质代谢的酶和基因的活性和mRNA表达。Cu暴露降低了第21天和第42天的WG和SGR。铜暴露增加了肠道铜和脂质含量。Cu积累增加是由于Cu- atpase和Cu、Zn-SOD酶活性以及参与Cu转运的基因（CTR1、CTR2、DMT1、ATP7a、ATP7b、MTI和MT2）表达增加所致。水中Cu暴露也增加了脂肪生成酶（6PGD和ICDH在第21天和第42天，ME在第42天）的活性，提高了脂肪生成基因（G6PD、6PGD、ME、ICDH、FAS和ACCa）、脂肪分解基因（ACCb、CPT I和HSLa）和肠道脂肪酸摄取基因（IFABP和FATP4）的mRNA水平。脂解的上调可能是由于对铜暴露的反应增加了解毒和维持正常身体功能的代谢支出。同时，铜暴露增加了脂肪生成和脂肪酸摄取，尽管脂肪分解增加，但导致肠道内净脂质积累。据我们所知，这是第一个涉及肠道脂质代谢与肠道铜吸收在水中暴露后的报道，为研究鱼的铜毒性提供了新的见解和证据。(C) 2016 Elsevier B.V.版权所有
The present study was conducted to explore the effects of waterborne Cu exposure on intestinal Cu transport and lipid metabolism of Synechogobius hasta. S. hasta were exposed to 0, 0.4721 and 0.9442 mu M Cu, respectively. Sampling occurred on days 0, 21 and 42, respectively. Growth performance, intestinal lipid deposition, Cu content, and activities and mRNA expression of enzymes and genes involved in Cu transport and lipid metabolism were analyzed. Cu exposure decreased WG and SGR on days 21 and 42. Cu exposure increased intestinal Cu and lipid contents. Increased Cu accumulation was attributable to increased enzymatic activities (Cu-ATPase and Cu, Zn-SOD) and genes' (CTR1, CTR2, DMT1,ATP7a, ATP7b, MTI and MT2) expression involved in Cu transport. Waterborne Cu exposure also increased activities of lipogenic enzymes (6PGD and ICDH on both days 21 and 42, ME on day 42), up-regulated mRNA levels of lipogenic genes (G6PD, 6PGD, ME, ICDH, FAS and ACCa), lipolytic genes (ACCb, CPT I and HSLa) and genes involved in intestinal fatty acid uptake (IFABP and FATP4) on both days 21 and 42. The up-regulation of lipolysis may result from the increased metabolic expenditure for detoxification and maintenance of the normal body functions in a response to Cu exposure. Meantime, Cu exposure increased lipogenesis and fatty acid uptake, leading to net lipid accumulation in the intestine despite increased lipolysis. To our knowledge, this is the first report involved in intestinal lipid metabolism in combination with intestinal Cu absorption following waterborne Cu exposure, which provides new insights and evidence into Cu toxicity in fish. (C) 2016 Elsevier B.V. All rights reserved.