Inhibition of radical reaction of apolipoprotein B-100 and α-tocopherol in human plasma by green tea catechins
Inhibition of radical reaction of apolipoprotein B-100 and α-tocopherol in human plasma by green tea catechins
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DOI：
10.1021/jf000973i
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发表时间：
2000-12-01
影响因子：
6.1
通讯作者：
Kojo, S
中科院分区：
文献类型：
--
作者：
Hashimoto, R;Yaita, M;Kojo, S
（-）-表儿茶素（EC）、（-）-表没食子儿茶素（EGC）、（-）-表儿茶素没食子酸酯（ECg）、（-）-表没食子儿茶素没食子酸酯（EGCg）和Trolox抑制载脂蛋白B-100（apo B）和α-生育酚在由Cu 2+引发的人血浆自由基反应中的降低。EC、EGG、ECg、EGCg和Trolox对apoB断裂的50%抑制（IC 50）浓度分别为39.1、42.2、14.6、21.3和36.2 μ M。对于α-生育酚消耗，观察到类似的IC 50值，表明apoB片段化和α-生育酚消耗之间的密切关系。这些结果表明，茶儿茶素作为一种有效的抗氧化剂在血浆中，没食子酸酯基团具有很强的抗氧化活性。
(-)-Epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg), (-)-epigallocatechin gallate (EGCg), and Trolox inhibited the decreases of apolipoprotein B-100 (apoB) and alpha -tocopherol in a radical reaction of human plasma initiated by Cu2+. The concentrations of EC, EGG, ECg, EGCg, and Trolox for 50% inhibition (IC50) Of apoB fragmentation were 39.1, 42.2, 14.6, 21.3, and 36.2 muM respectively. Similar IC50 values were observed for cx-tocopherol consumption, indicating the close relationship between apoB fragmentation and alpha -tocopherol consumption. These results demonstrate that tea catechins serve as an effective antioxidant in plasma and that the gallate group has a strong antioxidative activity.