Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control.
Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control.
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DOI：
10.1155/2016/9641965
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Peng W
中科院分区：
文献类型：
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作者：
Xuan H;Wang Y;Li A;Fu C;Wang Y;Peng W
为了了解中国蜂胶水提取物（CPWE）抗肿瘤活性的物质基础，我们开发了一种简单高效的方法，采用大孔吸附树脂结合制备型高效液相色谱法，分离纯化了11种化学成分（咖啡酸、阿魏酸、异阿魏酸、3,4-二甲氧基肉桂酸、松班辛、咖啡酸苯甲酯、咖啡酸苯乙酯、芹菜素、松香素、白杨素和高良姜素）来自 CPWE；然后我们使用不同的人类肿瘤细胞系（MCF-7、MDA-MB-231、HeLa 和 A549）测试了这 11 种成分的抗肿瘤活性。此外，还研究了 CPWE 有效成分的细胞迁移、蛋白酶原 3 水平和活性氧 (ROS)。我们的数据显示 CPWE 的 11 种成分的抗肿瘤活性彼此不同。 CPWE及其有效成分通过抑制肿瘤细胞迁移、激活caspase 3、促进ROS产生来诱导细胞凋亡。由此可见，蜂胶的抗肿瘤作用并不依赖于单一成分，必定存在“生物活性成分”，这也为中药蜂胶的质量控制提供了新的思路。
To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin, and galangin) from CPWE; then we tested the antitumor activities of these eleven components using different human tumor cell lines (MCF-7, MDA-MB-231, HeLa, and A549). Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist “bioactive components,” which also provides a new idea for Chinese propolis quality control.