Influence of Cold Plasma Atmospheric Jet on Surface Integrin Expression of Living Cells
Influence of Cold Plasma Atmospheric Jet on Surface Integrin Expression of Living Cells
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DOI：
10.1002/ppap.200900086
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发表时间：
2010-03-22
影响因子：
3.5
通讯作者：
Keidar, Michael
中科院分区：
文献类型：
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作者：
Shashurin, Alexey;Stepp, Mary Ann;Keidar, Michael
研究了冷大气等离子体射流处理细胞引起的效应。通过延时显微镜测量细胞迁移率。为了表征细胞表面整联蛋白表达，通过流式细胞术测量表面整联蛋白用特异性抗体染色后细胞的荧光响应。我们表明用等离子体射流处理细胞在亚细胞水平上影响细胞，即降低细胞表面整合素的表达（测试了β（1）和α（v）整合素）。这种整合素表达的变化可能是在细胞水平上观察到的效应的原始原因，例如实验观察到的细胞迁移率降低和细胞脱离。
The effects induced in cells due to treatment with cold atmospheric plasma jet are studied. Cell migration rate is measured by means of time-lapse microscopy. In order to characterize cell surface integrin expression, the fluorescent response of cells after surface integrins are stained with specific antibodies is measured by flow cytometry. We show that treatment of cells with plasma jet affects the cells on sub-cellular level, namely decreases expression of cell surface integrins (beta(1) and alpha(v) integrins were tested). This change in integrin expression might be the original cause for the effects observed on cellular level, such as reduced cell migration rate and cell detachment observed experimentally.