Volatile anesthetic modulation of lung injury and outcome in a murine model of multiple organ dysfunction syndrome.
Volatile anesthetic modulation of lung injury and outcome in a murine model of multiple organ dysfunction syndrome.
复制标题
挥发性麻醉剂对多器官功能障碍综合征小鼠模型肺损伤和结果的调节。
作者：
J. Shayevitz;J. Rodríguez;L. Gilligan;K. Johnson;A. Tait
全身麻醉已被证明对炎症反应有显着影响。我们假设，肺病理生理学将在继发性多器官功能障碍综合征（MODS）引起的腹腔内酵母多糖盐水悬浮液的小鼠模型减弱。CD-1小鼠用含1%氟烷或1.5%异氟烷的30%氧气N2载气麻醉6小时。另一组小鼠仅暴露于N2载气中的30%氧气。通过测量肺髓过氧化物酶活性（中性粒细胞募集）来量化对酵母聚糖的炎症反应。通过测定肺对放射性白蛋白的渗透性程度（渗透性指数）来评估肺损伤。未麻醉的损伤小鼠在酵母多糖注射后2小时表现出最大的肺髓过氧化物酶活性（.671 +/- .07 Δ OD.min-1），其在用氟烷（.369 +/- .054）和异氟烷（.324 +/- .055）麻醉的损伤小鼠中显著减弱（p < .01）。在未麻醉的损伤小鼠中，最大肺渗透性指数出现在注射后8小时（.398 +/- .019），并且在用氟烷（.255 +/- .02）和异氟烷（.224 +/- .019）麻醉的损伤小鼠中减弱（p < .01）。组织学结果与定量髓过氧化物酶和渗透性指数值相对应。氟烷和异氟烷减轻多器官功能障碍综合征小鼠模型的肺部炎症和损伤。这种衰减可能与挥发性麻醉剂对炎症反应的调节有关。
General anesthesia has been shown to have a significant impact on the inflammatory response. We hypothesized that lung pathophysiology will be attenuated in a mouse model of secondary multiple organ dysfunction syndrome (MODS) elicited by intraperitoneal zymosan suspension in saline. CD-1 mice were anesthetized for 6 h with either 1% halothane or 1.5% isoflurane in 30% oxygen in N2 carrier gas. Another group of mice was exposed to 30% oxygen in N2 carrier gas only. The inflammatory response to zymosan was quantified by measuring lung myeloperoxidase activity (neutrophil recruitment). Lung injury was estimated by determining the degree of lung permeability to radioactive albumin (permeability index). Unanesthetized injured mice exhibited maximal lung myeloperoxidase activity 2 h after zymosan injection (.671 +/- .07 delta OD.min-1), which was significantly attenuated (p < .01) in injured mice anesthetized with halothane (.369 +/- .054) and isoflurane (.324 +/- .055). The maximum lung permeability index occurred 8 h after injection in the unanesthetized, injured mice (.398 +/- .019), and was attenuated (p < .01) in injured mice anesthetized with halothane (.255 +/- .02) and isoflurane (.224 +/- .019). Histopathological findings corresponded to the quantitative myeloperoxidase and permeability index values. Halothane and isoflurane attenuate lung inflammation and injury in this mouse model of multiple organ dysfunction syndrome. This attenuation may be related to modulation of the inflammatory response by volatile anesthetics.