Sensitive Determination Method for Mercury Ion, Methyl-, Ethyl-, and Phenyl-mercury in Water and Biological Samples Using High-Performance Liquid Chromatography with Chemiluminescence Detection
Sensitive Determination Method for Mercury Ion, Methyl-, Ethyl-, and Phenyl-mercury in Water and Biological Samples Using High-Performance Liquid Chromatography with Chemiluminescence Detection
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DOI：
10.2116/analsci.28.959
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发表时间：
2012-10-01
影响因子：
1.6
通讯作者：
Tomiyasu, Takashi
中科院分区：
文献类型：
--
作者：
Kodamatani, Hitoshi;Matsuyama, Akito;Tomiyasu, Takashi
建立了一种灵敏的汞形态分析方法。将汞离子、甲基汞、乙基汞和苯基汞与二硫代氨基甲酸乙美汀(Em etine-CS2)络合，然后进样到高效液相色谱仪和三(2，2‘-联吡啶)Ru(III)化学发光检测系统中。埃美汀-CS2络合剂除可作为分离检测试剂外，还可有效地用于浓度的测定。汞络合物的线性范围为0.050-10微克L(以汞计)。(埃美汀-CS2)(2)汞、埃美汀-CS2-甲基汞、埃米汀-CS2-乙基汞和埃米汀-CS2-苯基汞的检出限分别为30、17、21和22 ng L-1。该方法的灵敏度使水样中汞形态的测定达到亚ppb级。并将该方法应用于生物样品的酸浸和以二硫代乙胺为萃取剂的液-液萃取。测定结果与标准物质值吻合较好。
A sensitive determination method for mercury speciation analysis was developed. Four mercury species, mercury ion, methylmercury, ethylmercury, and phenylmercury, were complexed with emetine-dithiocarbamate (emetine-CS2), and then injected onto a HPLC instrument coupled with a tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection system. The emetine-CS2 complexing agent was effectively used to measure the concentration in addition to serving as a separation and detection reagent. The calibration curves for these mercury complexes were linear in the range of 0.050 - 10 mu g L-1 (as Hg). The limit of detection for (emetine-CS2)(2)Hg, emetine-CS2-methylmercury, emetine-CS2-ethylmercury, and emetine-CS2-phenylmercury were 30, 17, 21, and 22 ng L-1, respectively. The sensitivity of this method enables the determination of mercury species in water samples at sub-ppb levels. Furthermore, the method was applied to biological samples in combination with acid leaching and liquid-liquid extraction using emetine-CS2 as an extraction reagent. The determination results were in good agreement with the values of the certified reference materials.