Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat
Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat
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DOI：
10.1128/aem.01352-07
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发表时间：
2007-10-01
影响因子：
4.4
通讯作者：
Duran, Robert
中科院分区：
文献类型：
--
作者：
Bordenave, Sylvain;Goni-Urriza, Maria Soledad;Duran, Robert
研究了石油污染对Salins-de-Giraud（Camargue，法国）原始微生物垫细菌群落的影响。垫子被保持为微观世界，并被Erika残骸中的2号燃料油污染。复杂的细菌群落的演变进行了监测，结合分析的基础上16 S rRNA基因和它们的转录本。基于16 S rRNA基因的末端限制性片段长度多态性（T-RFLP）分析清楚地显示了孵育60天后重质燃料油的影响。在实验结束时，恢复了最初的群落结构，说明了这种微生物生态系统的恢复能力。此外，通过T-RFLP和基于16 S rRNA的克隆文库分析来评估代谢活性细菌群落的响应。在将重质燃料油加入到微观世界中之后，活性细菌群落的结构立即被修改，这表明快速的微生物垫响应。γ-变形菌的成员最初在受污染的微观世界中占主导地位。假单胞菌属和不动杆菌属是该纲的主要代表属。经过90天的培养，γ变形菌被“杆菌”和α变形菌取代。这项研究显示了污染后不同时间段微生物垫群落发生的主要变化。在实验结束时，RNA方法还证明了微生物垫群落在抵抗石油污染造成的环境压力方面的弹性。
The effects of petroleum contamination on the bacterial community of a pristine microbial mat from Salins-de-Giraud (Camargue, France) have been investigated. Mats were maintained as microcosms and contaminated with no. 2 fuel oil from the wreck of the Erika. The evolution of the complex bacterial community was monitored by combining analyses based on 16S rRNA genes and their transcripts. 16S rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) analyses clearly showed the effects of the heavy fuel oil after 60 days of incubation. At the end of the experiment, the initial community structure was recovered, illustrating the resilience of this microbial ecosystem. In addition, the responses of the metabolically active bacterial community were evaluated by T-RFLP and clone library analyses based on 16S rRNA. Immediately after the heavy fuel oil was added to the microcosms, the structure of the active bacterial community was modified, indicating a rapid microbial mat response. Members of the Gammaproteobacteria were initially dominant in the contaminated microcosms. Pseudomonas and Acinetobacter were the main genera representative of this class. After 90 days of incubation, the Gammaproteobacteria were superseded by "Bacilli" and Alphaproteobacteria. This study shows the major changes that occur in the microbial mat community at different time periods following contamination. At the conclusion of the experiment, the RNA approach also demonstrated the resilience of the microbial mat community in resisting environmental stress resulting from oil pollution.