AEDES-TRISERIATUS (DIPTERA, CULICIDAE) AND LACROSSE VIRUS .3. ENHANCED ORAL-TRANSMISSION BY NUTRITION-DEPRIVED MOSQUITOS
AEDES-TRISERIATUS (DIPTERA, CULICIDAE) AND LACROSSE VIRUS .3. ENHANCED ORAL-TRANSMISSION BY NUTRITION-DEPRIVED MOSQUITOS
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DOI：
10.1093/jmedent/21.3.249
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发表时间：
1984-01-01
影响因子：
2.1
通讯作者：
HARAMIS, LD
中科院分区：
文献类型：
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作者：
GRIMSTAD, PR;HARAMIS, LD
用口服La Crosse(LAC)病毒的方法，饲养了3种不同体型[小(S)、正常(N)和大(L)]的成虫。在所有试验中，作为幼虫(S级)承受最严重营养剥夺的雌性蚊子传播LAC病毒的比率明显高于中度剥夺N级和非剥夺L级的蚊子(平均传播率分别为82%、54%和52%)。虽然S级蚊子摄取的传染病血粉与干重的比例高于其他两级蚊虫，但实际摄取的病毒量在3个大小级蚊虫中仅相差0.4log10个空斑单位/0.1mg干重。虽然口腔传播的增强可能在一定程度上与剂量有关，但同样明显的是，作为幼虫缺乏营养的成年蚊子对LAC病毒感染的生理抵抗力可能低于缺乏营养的个体。在实验室用最佳幼虫饲料饲养的成年蚊子进行的病毒传播实验可能不能反映野外种群中的媒介能力。因此，在以前的许多研究中，可能严重低估了重要的流行病学变量，如口腔传播率。
Adult A. triseriatus, reared as larvae under a dietary regimen that produced 3 distinct body size classes [small (S), normal (N), and large (L)], were orally infected with La Crosse (LAC) virus. In all trials female mosquitoes that had endured the severest nutritional deprivation as larvae (S class) transmitted LAC virus at rates considerably higher than the mosquitoes from the moderately deprived N and nondeprived L classes (mean transmission rates of 82, 54 and 52%, respectively). While the S class mosquitoes ingested a proportionally larger infectious blood meal in relation to dry weight than the other 2 classes, the actual amount of virus ingested varied only about 0.4 log10 plaque-forming units/0.1 mg dry body weight among the 3 size classes. While enhanced oral transmission may be dose related to some extent, it is also apparent that adult mosquitoes nutritionally deprived as larvae may be physiologically less resistant to infection by LAC virus than less deprived individuals. Virus transmission experiments conducted with adult mosquitoes reared on optimal larval diets in the laboratory may not reflect the vector capacity found in field populations. Consequently, epidemiologically important variables such as oral transmission rates may have been seriously underestimated in many previous studies.