Structural determination and pro-angiogenic effect of polysaccharide from the pollen of Typha angustifolia L.
Structural determination and pro-angiogenic effect of polysaccharide from the pollen of Typha angustifolia L.
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DOI：
10.1016/j.ijbiomac.2022.10.002
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发表时间：
2022-10
影响因子：
8.2
通讯作者：
Mingliang Gao;Jinshan Lan;Yuling Zha;Weifeng Yao;Beihua Bao;Mingqiu Shan;Fang Zhang;Gui-Sheng Zhou;Sheng Yu;Fang-Fang Cheng-Fang;Yudan Cao;Hui Yan;Li Zhang;Pei-dong Chen
中科院分区：
文献类型：
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作者：
Mingliang Gao;Jinshan Lan;Yuling Zha;Weifeng Yao;Beihua Bao;Mingqiu Shan;Fang Zhang;Gui-Sheng Zhou;Sheng Yu;Fang-Fang Cheng-Fang;Yudan Cao;Hui Yan;Li Zhang;Pei-dong Chen
从狭叶香蒲（TyphaangustifoliaL.）花粉中分离纯化了四个多糖组分（TPP-1、TPP-2、TPP-3和TPP-4），并通过高效凝胶渗透色谱（HPGPC）、单糖组成分析、甲基化分析和核磁共振（NMR）等手段对TPP-3的结构进行了进一步的鉴定。TPP-3是一种均一的杂多糖，平均分子量为5.5 × 104 Da，由8种单糖组成。TPP-3的促血管生成活性在HUVECs和VEGFR酪氨酸激酶抑制剂II（VRI）诱导的血管缺陷斑马鱼模型上得到验证。其促血管生成活性与VEGF/PI 3 K/Akt信号通路的激活密切相关。
Four fractions of polysaccharides (TPP-1, TPP-2, TPP-3, and TPP-4) were isolated and purified from the pollen ofTypha angustifoliaL., and the structure of TPP-3 was furtherly determined by HPGPC (High Performance Gel Permeation Chromatography), monosaccharide composition analysis, methylation analysis and NMR (Nuclear Magnetic Resonance). TPP-3 was found to be a homogeneous heteropolysaccharide with an average molecular weight of 5.5 × 104Da and composed of eight types of monosaccharides. The pro-angiogenic activities of TPP-3 were verified on HUVECs and VEGFR tyrosine kinase inhibitor II (VRI)-induced vascular defect zebrafish model. Furthermore, the underlying mechanism investigation showed that its pro-angiogenic activities were closely related with the activation of VEGF/PI3K/Akt signaling pathway.