Nop2 is required for mammalian preimplantation development.
Nop2 is required for mammalian preimplantation development.
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DOI：
10.1002/mrd.22600
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发表时间：
2016-02
影响因子：
2.5
通讯作者：
Mager J
中科院分区：
文献类型：
--
作者：
Cui W;Pizzollo J;Han Z;Marcho C;Zhang K;Mager J
核仁蛋白2（Nucleolar protein 2，NOP 2）是从酵母到人类进化上保守的蛋白质，在促进细胞增殖、细胞周期进程和肿瘤侵袭性等方面发挥重要作用。然而，Nop 2在早期哺乳动物胚胎发育过程中的表达模式和功能尚未研究。我们在小鼠植入前胚胎中进行基于RNA干扰（RNAi）的筛选时，将Nop 2确定为发育到囊胚阶段的必需基因。Nop 2在整个植入前发育过程中表达，在8细胞和桑椹胚阶段分别具有最高的mRNA和蛋白质积累。RNAi介导的Nop 2敲低导致胚胎停滞为桑椹胚。NOP 2缺陷的胚胎表现出卵裂球数量减少，细胞凋亡大大增加，细胞谱系特化受损。此外，Nop 2的敲除导致所有RNA种类的总体减少，包括rRNA、小核RNA、小核仁RNA和mRNA。总而言之，我们的结果表明Nop 2是囊胚形成的必需基因，并且是小鼠植入前胚胎发育期间RNA加工和/或体内稳定性所需的。
Nucleolar protein 2 (NOP2) is evolutionarily conserved from yeast to human, and has been found to play an important role in accelerating cell proliferation, cell-cycle progression, and tumor aggressiveness. The expression pattern and function of Nop2 during early mammalian embryo development, however, has not been investigated. We identified Nop2 as an essential gene for development to the blastocyst stage while performing an RNA interference (RNAi)-based screen in mouse preimplantation embryos. Nop2 is expressed throughout preimplantation development, with highest mRNA and protein accumulation at the 8-cell and morula stages, respectively. RNAi-mediated knockdown of Nop2 results in embryos that arrest as morula. NOP2-deficient embryos exhibit reduced blastomere numbers, greatly increased apoptosis, and impaired cell-lineage specification. Furthermore, knockdown of Nop2 results in global reduction of all RNA species, including rRNA, small nuclear RNA, small nucleolar RNA, and mRNA. Taken together, our results demonstrate that Nop2 is an essential gene for blastocyst formation, and is required for RNA processing and/or stability in vivo during preimplantation embryo development in the mouse.