SPECTRAL EVIDENCE FOR INTERACTIONS BETWEEN MEMBRANE-BOUND HEMES - RESONANCE RAMAN-SPECTRA OF MITOCHONDRIAL CYTOCHROME-B-C1 COMPLEX AS A FUNCTION OF REDOX POTENTIAL
SPECTRAL EVIDENCE FOR INTERACTIONS BETWEEN MEMBRANE-BOUND HEMES - RESONANCE RAMAN-SPECTRA OF MITOCHONDRIAL CYTOCHROME-B-C1 COMPLEX AS A FUNCTION OF REDOX POTENTIAL
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DOI：
10.1016/0014-5793(77)80438-9
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发表时间：
1977-01-01
期刊：
影响因子：
3.5
通讯作者：
ERECINSKA, M
中科院分区：
文献类型：
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作者：
ADAR, F;ERECINSKA, M
自从Spiro等人对细胞色素c中由激光频率和卟啉rr到n* 跃迁之间的共振增强的散射进行初步分析以来，已经报道了许多血红素蛋白[1-lo]和金属卟啉[1 -161]的共振拉曼（RR）光谱。[17]第10段。后来，对细胞色素c [18-201]和其他金属卟啉[15，16，2 l]中的散射现象进行了更详细的描述，并在RR带频率与各种血红素蛋白中铁的氧化和自旋状态之间进行了相关性[6，22，23]。此外，有人提出，由于其高分辨率能力，RR光谱可以用作功能生物膜中血红素之间相互作用的探针[24]。对四苯基卟啉~-0x0二聚体的模型研究表明，该技术确实对血红素聚集敏感，我们对琥珀酸细胞色素c还原酶的初步工作表明，由于各种原因，它是一个富有成效的RR研究系统：（1）分离的细胞色素的RR光谱显示出明确的标记带[7];（2）亚铁细胞色素的RR量子产率异常大（lo-'）[25];（3）亚铁细胞色素中RR效应的光谱表征是高度发达的[17-191]。
Resonance Raman (RR) spectra of many hemeproteins [l-lo] and metalloporphyrins[1 l-161 have been reported since the initial analysis of the scattering enhanced by resonance between the laser frequency and porphyrin rr to n* transitions in cytochrome c by Spiro et al.[17]. Later, more detailed descriptions of the scattering phenomenon in both cytochrome c [18-201 and other metalloporphyrins [15, 16, 2 l] were presented and correlations were made between RR band frequencies and oxidation and spin states of the iron in various hemeproteins [6, 22, 23]. Moreover, the proposal was made that because of its high resolution capabilities, RR spectra could be exploited as a probe of interactions between hemes in functioning biological membranes [24]. A model study on the~-0x0 dimer of tetraphenylporphin indicated that this technique was indeed sensitive to heme aggregation.Our initial work on the succinate cytochrome c reductase showed it to be a fruitful system for RR study for a variety of reasons:(1) RR spectra of isolated cytochromes exhibit well-defined marker bands [7];(2) The RR quantum yields of ferrous cytochromes are unusually large (lo-‘)[25];(3) The spectroscopic characterization of the RR effect in ferrous cytochromes is highly developed [17-191.