Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage.
Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage.
复制标题
碱基切除 DNA 修复基因的表达作为氧化 DNA 损伤的生物标志物。
DOI：
10.1016/j.canlet.2004.12.002
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Rusyn,Ivan
中科院分区：
文献类型：
--
作者：
Powell,ChristineL;Swenberg,JamesA;Rusyn,Ivan
Oxidative stress induced DNA damage is considered to be the most common insult affecting the genome. Moreover, it is recognized as a common pathway to mutations and is suggested to play a major role in the development of chronic diseases such as cancer. However, current analytical methods used to detect oxidative DNA damage have been hampered by both technical and biological obstacles. These include spurious oxidation during DNA isolation and processing, and the inherent removal of damaged bases by numerous operating DNA repair systems. The removal of oxidized bases is performed predominantly by the base excision repair (BER) pathway and it has been shown that induction of DNA repair genes occurs in response to oxidative stress. Here, we demonstrate the utility of measuring changes in expression of BER genes as a sensitive in vivo biomarker for oxidative DNA damage.
