Growth of linear spicules in cultured primary mesenchymal cells of sea urchin embryos is bidirectional.
Growth of linear spicules in cultured primary mesenchymal cells of sea urchin embryos is bidirectional.
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培养的海胆胚胎原代间充质细胞中的线性针状体的生长是双向的。
DOI：
10.1016/0012-1606(88)90152-2
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发表时间：
1988
影响因子：
2.7
通讯作者：
Lennarz,WJ
中科院分区：
文献类型：
--
作者：
Decker,GL;Lennarz,WJ
在一个简单的体外培养系统中研究了海胆胚胎骨针的生长，在该系统中，初级间充质细胞附着于基质，通过丝状伪足迁移和融合，随后存款CaCO 3，在大多数情况下是以线性杆的形式。45 Ca ~（2+）脉冲追踪标记后的放射自显影显示，在这样一个系统中，形成的线状针状体有两个生长焦点。通过向每个杆的两端添加大约等量的Ca 2+发生伸长。具有可变数量的伸长部位（尖端）的多形骨针也表现出类似的Ca 2+沉积模式。因此，线性和相对复杂的骨骼形态的生长显然是由相同的基本机制完成的。
The growth of spicules of the sea urchin embryo was studied in a simplein vitrosystem in which primary mesenchymal cells attach to the substrata, migrate and fuse via filopodia, and subsequently deposit CaCO3, which in most cases is in the form of linear rods. The use of autoradiographs following45Ca2+pulse-chase labeling revealed that in such a system linear spicules that formed had two focal points of growth. Elongation occurred by addition of approximately equivalent amounts of Ca2+to both ends of each rod. Multiform spicules having variable numbers of elongation sites (tips) also showed a similar pattern of Ca2+deposition. Thus, the growth of both linear and relatively complex skeletal forms is apparently accomplished by the same basic mechanism.