The presence of centrioles in artificially activated sea urchin eggs.
The presence of centrioles in artificially activated sea urchin eggs.
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人为激活的海胆卵中的中心元素存在。
DOI：
10.1083/jcb.11.1.244
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发表时间：
1961-10
期刊：
影响因子：
--
通讯作者：
DIRKSEN, E R
中科院分区：
文献类型：
--
作者：
DIRKSEN, E R
方法通过对Loeb（1913）的“双重方法”的改进，人工激活加利福尼亚海岸海胆（Strongylocentrotus purpuratus）的segs。在任何时候都采取适当的预防措施，防止精子污染。每25毫升蛋悬液中加入1毫升0.11 N丁酸，发酵2分钟。经过这种治疗
METHODSEggs of the California coast sea urchin, Strongylocentrotus purpuratus, were artificially activated by a modification of the" double method" of Loeb (1913). At all times suitable precautions were maintained against contamination with sperm. To every 25 cc of egg suspension, 1 cc of 0.11 N butyric acid was added for a period of 2 minutes. After this treatment