Alterations in mesenteric lymph node T cell phenotype and cytokine secretion are associated with changes in thymocyte phenotype after LP-BM5 retrovirus infection.
Alterations in mesenteric lymph node T cell phenotype and cytokine secretion are associated with changes in thymocyte phenotype after LP-BM5 retrovirus infection.
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LP-BM5逆转录病毒感染后肠系膜淋巴结T细胞表型和细胞因子分泌的变化与胸腺细胞表型的变化相关。
DOI：
10.1080/17402520500303339
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发表时间：
2005
影响因子：
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通讯作者：
Watson,RonaldR
中科院分区：
文献类型：
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作者：
Lopez,MariaC;Watson,RonaldR
本研究以Lp-BM5逆转录病毒感染晚期的小鼠MLN细胞和胸腺细胞为研究对象。IL-7+细胞百分率下降，IL-16+细胞百分率升高，提示Lp-BM5感染后这些细胞因子的分泌也发生了改变。表达IL-7受体的mln T细胞百分率显著降低，表达TNFR-p75的mln T细胞百分率和表达TNFR-p55的B细胞百分率显著升高。同时对细胞表面标志物和细胞因子的分泌进行分析，试图了解干扰素-Υ分泌的解除是否可以归因于特定的细胞表型，结论是所有研究的T细胞亚群在逆转录病毒感染后都增加了干扰素-Υ的分泌。最后，进一步分析胸腺细胞表型，试图将胸腺细胞表型的变化与MLN细胞表型的变化联系起来。结果表明，未成熟(CD3-)和成熟(CD3+)单项阳性胸腺细胞的增加是由于未成熟(CD3-)和成熟(CD3+)单项阳性胸腺细胞的增加。此外，单个阳性成熟胸腺细胞的表型与以前在mln T细胞上看到的表型相似。综上所述，我们可以得出结论，LP-BM5利用免疫系统到达胸腺，在那里它干扰了功能成熟T细胞的产生，有利于表型异常的T细胞的发育。这些新的T细胞被激活以分泌几种细胞因子，这些细胞因子反过来将有利于逆转录病毒的复制，并抑制免疫系统控制感染的任何尝试。
In this study, mouse MLN cells and thymocytes from advanced stages of LP-BM5 retrovirus infection were studied. A decrease in the percentage of IL‐7+cells and an increase in the percentage of IL‐16+cells in the MLN indicated that secretion of these cytokines was also altered after LP‐BM5 infection. The percentage of MLN T cells expressing IL‐7 receptors was significantly reduced, while the percentage of MLN T cells expressing TNFR‐p75 and of B cells expressing TNFR‐p55 increased. Simultaneous analysis of surface markers and cytokine secretion was done in an attempt to understand whether the deregulation of IFN‐Υ secretion could be ascribed to a defined cell phenotype, concluding that all T cell subsets studied increased IFN‐Υ secretion after retrovirus infection. Finally, thymocyte phenotype was further analyzed trying to correlate changes in thymocyte phenotype with MLN cell phenotype. The results indicated that the increase in single positive either CD4+CD8-or CD4-CD8+cells was due to accumulation of both immature (CD3-) and mature (CD3+) single positive thymocytes. Moreover, single positive mature thymocytes presented a phenotype similar to the phenotype previously seen on MLN T cells. In summary, we can conclude that LP‐BM5 uses the immune system to reach the thymus where it interferes with the generation of functionally mature T cells, favoring the development of T cells with an abnormal phenotype. These new T cells are activated to secrete several cytokines that in turn will favor retrovirus replication and inhibit any attempt of the immune system to control infection.