P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb
P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb
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DOI：
10.1007/s11302-011-9286-z
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发表时间：
2012-06-01
影响因子：
3.5
通讯作者：
Hirnet, Daniela
中科院分区：
文献类型：
--
作者：
Fischer, Timo;Rotermund, Natalie;Hirnet, Daniela
最近已经表明，腺苷-5 '-三磷酸（ATP）与谷氨酸一起从嗅球中的感觉轴突释放，在那里它刺激神经胶质细胞中的钙信号传导，而在嗅球中尚未记录已鉴定的神经元对ATP的反应。我们使用笼状ATP的光解引起ATP的快速上升，并在急性小鼠脑切片中测量二尖瓣细胞（嗅球的输出神经元）的全细胞电流反应。笼状ATP的宽场光解引起二尖瓣细胞突触输入的增加，表明ATP依赖性网络活动的增加。突触活动的增加伴随着钙瞬变的树突状簇的二尖瓣细胞，共聚焦钙成像测量。ATP对神经网络活动的刺激作用可被笼状腺苷5 '-二磷酸的光释放所模拟，并可被P2 Y（1）受体拮抗剂MRS 2179所抑制。记录的二尖瓣细胞的树突簇支配的肾小球中的笼状ATP的局部光解引起的电流类似于宽场照明引起的电流。结果表明，肾小球中P2 Y（1）受体的激活可刺激嗅球中的网络活动。
It has recently been shown that adenosine-5'-triphosphate (ATP) is released together with glutamate from sensory axons in the olfactory bulb, where it stimulates calcium signaling in glial cells, while responses in identified neurons to ATP have not been recorded in the olfactory bulb yet. We used photolysis of caged ATP to elicit a rapid rise in ATP and measured whole-cell current responses in mitral cells, the output neurons of the olfactory bulb, in acute mouse brain slices. Wide-field photolysis of caged ATP evoked an increase in synaptic inputs in mitral cells, indicating an ATP-dependent increase in network activity. The increase in synaptic activity was accompanied by calcium transients in the dendritic tuft of the mitral cell, as measured by confocal calcium imaging. The stimulating effect of ATP on the network activity could be mimicked by photo release of caged adenosine 5'-diphosphate, and was inhibited by the P2Y(1) receptor antagonist MRS 2179. Local photolysis of caged ATP in the glomerulus innervated by the dendritic tuft of the recorded mitral cell elicited currents similar to those evoked by wide-field illumination. The results indicate that activation of P2Y(1) receptors in the glomerulus can stimulate network activity in the olfactory bulb.