Femtosecond stimulated Raman study of the photoactive flavoprotein AppABLUF
Femtosecond stimulated Raman study of the photoactive flavoprotein AppABLUF
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DOI：
10.1016/j.cplett.2017.03.030
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发表时间：
2017-09-01
影响因子：
2.8
通讯作者：
Meech, Stephen R.
中科院分区：
文献类型：
--
作者：
Hall, Christopher R.;Heisler, Ismael A.;Meech, Stephen R.
利用飞秒受激拉曼光谱（FSRS）研究了黄素（BLUF）结构域光感受器AppA（BLUF）的蓝光光循环。结果表明，FSRS光谱对蛋白质的光适应状态非常敏感，可以探测蛋白质的激发态动力学。对最敏感的激发态拉曼活性模式的主要贡献来自黄素环模式。然而，激发态结构的TD-DFT计算表明，实验观察到的光谱位移的再现和分配将需要对黄素在其特定蛋白质环境中的高水平计算。(C) 2017 Elsevier B.V.版权所有
Femtosecond stimulated Raman spectroscopy (FSRS) is applied to study the photocycle of a blue light using flavin (BLUF) domain photoreceptor, AppA(BLUF). It is shown that FSRS spectra are sensitive to the light adapted state of the protein and probe its excited state dynamics. The dominant contribution to the most sensitive excited state Raman active modes is from flavin ring modes. However, TD-DFT calculations for excited state structures indicate that reproduction and assignment of the experimentally observed spectral shift will require high level calculations on the flavin in its specific protein environment. (C) 2017 Elsevier B.V. All rights reserved.