Raman and SERS recognition of β-carotene and haemoglobin fingerprints in human whole blood
Raman and SERS recognition of β-carotene and haemoglobin fingerprints in human whole blood
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DOI：
10.1016/j.saa.2011.03.048
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发表时间：
2011-09-01
影响因子：
4.4
通讯作者：
Zerbi, Giuseppe
中科院分区：
文献类型：
--
作者：
Casella, Michele;Lucotti, Andrea;Zerbi, Giuseppe
目前的工作报告了新鲜全血（即血液测试后）中 β-胡萝卜素和血红蛋白在不同激光激发（即可见光（514 nm）和近红外（NIR，785 nm））下的拉曼和表面增强拉曼散射（SERS）振动指纹。胶体银纳米颗粒的使用显着增强了拉曼信号，从而提供了清晰的血液 SERS 光谱。对收集到的光谱进行了检查，并发现了 β-胡萝卜素和血红蛋白血红素辅基的标记带。特别是，识别并指定了 β-胡萝卜素（514 nm 激发）、血液蛋白和血红素分子（785 nm 激发）的基本特征。此外，可以识别血红蛋白 SERS 信号并将其与其氧合状态（氧合血红蛋白）相关联。报告的数据显示了拉曼和 SERS 技术在无需预处理的情况下检测全血样本中重要生物分子的前景。 (C) 2011 Elsevier B.V. 保留所有权利。
The present work reports on Raman and Surface Enhanced Raman Scattering (SERS) vibrational fingerprints of beta-carotene and haemoglobin in fresh whole blood (i.e. right after blood test) with different laser excitations, i.e. visible (514 nm) and near-infrared (NIR, 785 nm). The use of colloidal silver nanoparticles significantly increases the Raman signal, thus providing a clear SERS spectrum of blood. The collected spectra have been examined and marker bands of beta-carotene and of the haem prosthetic group of haemoglobin have been found.In particular, the fundamental features of beta-carotene (514 nm excitation), blood proteins and haem molecules (785 nm excitation) were recognized and assigned. Moreover haemoglobin SERS signals can be identified and related with its oxygenation state (oxy-haemoglobin). The data reported show the prospects of Raman and SERS techniques to detect important bio-molecules in a whole blood sample with no pre-treatment. (C) 2011 Elsevier B.V. All rights reserved.