RELEASE OF CARBOXYMETHYL-CELLULASE AND BETA-GLUCOSIDASE FROM CELL-WALLS OF TRICHODERMA-REESEI
RELEASE OF CARBOXYMETHYL-CELLULASE AND BETA-GLUCOSIDASE FROM CELL-WALLS OF TRICHODERMA-REESEI
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DOI：
10.1007/bf00500103
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
KUBICEK, CP
中科院分区：
文献类型：
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作者：
KUBICEK, CP
测定了在纤维素和纤维素二糖上培养的里氏木霉QM 9414的胞底、细胞壁和胞外培养液中羧甲基纤维素酶和β-葡萄糖苷酶的活性。通过将羧甲基纤维素作为纤维素结合的cm -纤维素酶和β-葡萄糖苷酶的脱附物，发现这些酶在碳源消耗过程中与细胞壁结合，但在随后的培养阶段被排出体外。用各种化学试剂处理完整的细胞壁不能引起酶的释放。α-甘露聚糖酶或胰蛋白酶处理完整细胞壁释放cm -纤维素酶，而层次胺酶或几丁质酶处理完整细胞壁释放β-葡萄糖苷酶。这两种酶在磷酸盐缓冲液的自溶过程中也被释放。这种自溶伴随着细胞外甘露聚糖酶、层压板酶和蛋白酶的出现。结果表明，某些细胞壁聚合物的化学键的断裂。芦苇可能是导致生长后期培养液中出现cm -纤维素酶和β-葡萄糖苷酶的原因。
Carboxymethyl-cellulase and β-glucosidase activities were determined in the cytosole, cell walls and extracellular culture fluid ofTrichoderma reesei QM 9414cultivated on cellulose and cellobiose. By means of carboxymethylcellulose as a specific desorbens for cellulose bound CM-cellulase and β-glucosidase it was found that these enzymes are cell wall bound during consumption of the carbon source, but are excreted during the subsequent cultivation stage. Treatment of intact cell walls with various chemical agents could not cause a release of the enzyme. Treatment of intact cell walls with α-mannanase or trypsin released CM-cellulase, whereas, treatment with laminarinase or chitinase released β-glucosidase. Both enzymes were also released during autolysis in phosphate buffer. This autolysis was accompanined by the appearance of extracellular mannanase, laminarinase and proteinase. The results suggest that cleavage of chemical bonds of certain cell wall polymers ofT. reeseicould be responsible for the appearance of CM-cellulase and β-glucosidase in the culture fluid during later stages of growth.