Fluorescent and Opt-Electric Recording Bacterial Identification Device for Ultrasensitive and Specific Detection of Microbials.
Fluorescent and Opt-Electric Recording Bacterial Identification Device for Ultrasensitive and Specific Detection of Microbials.
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荧光和光电记录细菌鉴定装置，用于微生物的超灵敏和特异性检测。
DOI：
10.1021/acssensors.0c02007
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发表时间：
2020-12
期刊：
影响因子：
8.9
通讯作者：
Jiaomei Huang;Yongjie Zhong;Wenxing Li;Wenxia Wang;Chaoyang Li;Aimin Wang;Hong Yan;Y. Wan;Jinghong Li
中科院分区：
文献类型：
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作者：
Jiaomei Huang;Yongjie Zhong;Wenxing Li;Wenxia Wang;Chaoyang Li;Aimin Wang;Hong Yan;Y. Wan;Jinghong Li
微生物检测是食源性疾病防控的重要方面，需要一种灵敏度高、特异性强、及时性的理想检测系统。在此，我们提出了一种荧光光电记录细菌识别装置（FORBID），通过整合微生物的代谢特征和选择性底物催化作用，对微生物进行全自动实时光电传感分析。它简化了测试过程（一步）并减少了对专业技术人员的需求。此外，该系统对大肠杆菌和铜绿假单胞菌均表现出超灵敏（1 CFU/mL）和特异性检测（99%）。更重要的是，这个系统的时效性甚至比传统的培养方法还要好。相信该系统可以扩展到其他微生物的检测，并为病原体的检测提供潜在的替代方案。
Since microbial detection is an important aspect for the prevention and control of foodborne diseases, an ideal detection system with high sensitivity, strong specificity, and timeliness is needed. Here, we proposed a fluorescent and opt-electric recording bacterial identification device (FORBID) for fully automatic real-time photoelectric sensing analysis of microbials by integrating the metabolic characteristics of microbial and selective substrate catalysis. It simplifies the testing process (one-step) and decreases the need of professional technicians. Besides, the system exhibits ultrasensitive (1 CFU/mL) and specific detection (99%) in both microbials, Escherichia coli and Pseudomonas aeruginosa. More importantly, the timeliness of this system is even better than that of the traditional culture methods. It is believed that this system can be extended to the detection of other microorganisms and provide a potential alternative for the detection of pathogens.